Multiuser facility for flow cytometry and sorting

用于流式细胞术和分选的多用户设施

• 批准号: 7838662
• 负责人: WALTER J ESSELMAN
• 金额: $ 59.97万
• 依托单位: MICHIGAN STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2013-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7838662
• 关键词: 15 year old; Adenoviruses; Antigens; Area; Bacteria; Biohazardous Substance; Cancer Immunology Science; Cell Separation; Cells; Communities; DNA; Dengue; Development; Environment; Equipment; Flow Cytometry; Fluorescent Probes; Funding; HIV; Health Sciences; Human; Individual; Influenza; Label; Life; Maintenance; Manufacturer Name; Methods; Molecular Profiling; Performance; Property; Proteins; Regenerative Medicine; Research; Research Personnel; Research Project Grants; Sorting - Cell Movement; Speed; Staging; Stem cells; Sterility; United States National Institutes of Health; base; cell fixing; cell type; improved; instrument; microbiome; protein expression; research study

DESCRIPTION (provided by applicant): Flow cytometry is an automated method for the analysis and sorting of live or fixed cells based on size and fluorescent intensity using multiple fluorescent probes that label cells based on intrinsic properties (e.g. DNA) or expression of unique protein antigens. Fluorescent activated cell sorters allow for the isolation of individual live cells (sorting) based on their antigen or protein expression profiles. Unique and rare cell types, such as stem cell or cells in specific developmental stages, can be isolated sterilely from mixtures of cells and characterized or cultured. Flow cytometry has become critical to many areas of health sciences - including cancer, immunology and regenerative medicine. The objective of this proposal is to provide state-of-the-art flow cytometry and cell sorting to a large group of NIH funded investigators. In addition, the equipment will benefit a larger group of users who perform occasional flow cytometry experiments or cell isolations. We will replace a fifteen year-old BD Vantage cell sorter instrument whose maintenance is no longer supported by the manufacturer. This speed and performance of this new iCyt flow cytometer is much improved over the Vantage sorter it is replacing, and it has additional capability to carry out sterile sorting in a biocontainment hood, a feature that greatly increases its utility to the MSU the research community. Twelve individual research projects are described that range from sorting HIV, influenza, dengue and adenovirus infected cells; to the isolation of bacteria from the human gut microbiome. Since the proposed instrument is completely contained in a laminar flow environment, it will be capable of biohazard isolation and enforcement of strict sterility.

描述（由申请人提供）：流式细胞术是一种根据大小和荧光强度对活细胞或固定细胞进行分析和分选的自动化方法，使用多种荧光探针，根据固有特性（例如 DNA）或独特蛋白质抗原的表达来标记细胞。荧光激活细胞分选仪可以根据抗原或蛋白质表达谱分离单个活细胞（分选）。独特和稀有的细胞类型，例如干细胞或处于特定发育阶段的细胞，可以从细胞混合物中无菌分离并进行表征或培养。流式细胞术对于健康科学的许多领域都至关重要，包括癌症、免疫学和再生医学。该提案的目的是为一大批 NIH 资助的研究人员提供最先进的流式细胞术和细胞分选。此外，该设备将使更多偶尔进行流式细胞术实验或细胞分离的用户受益。我们将更换已有 15 年历史的 BD Vantage 细胞分选仪，其制造商不再支持其维护。这种新型 iCyt 流式细胞仪的速度和性能比它所取代的 Vantage 分选仪有了很大提高，并且它具有在生物防护罩中进行无菌分选的附加功能，这一功能大大增加了其对 MSU 研究界的实用性。描述了十二个单独的研究项目，范围包括分选艾滋病毒、流感、登革热和腺病毒感染的细胞；从人类肠道微生物组中分离细菌。由于所提出的仪器完全包含在层流环境中，因此它将能够隔离生物危害并执行严格的无菌操作。