GLYCOPROTEIN ALTERATIONS IN DIFFERENTIATION

分化过程中糖蛋白的变化

• 批准号: 3288986
• 负责人: WALTER J ESSELMAN
• 金额: $ 10.85万
• 依托单位: MICHIGAN STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-07-01 至 1989-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3288986
• 关键词: T lymphocyte; affinity chromatography; amines; carbohydrate structure; cell differentiation; cell sorting; clone cells; fluorescent dye /probe; gel filtration chromatography; helper T lymphocyte; lactose; leukocyte activation /transformation; lymphocyte; membrane activity; oligosaccharides

The overall goal of this study is to define the biochemical alterations that occur in oligosaccharides of cell surface glycoproteins during differentiation and during the functional expression of T lymphocytes and natural killer cells. The expression of N-linked oligosaccharides will be examined from whole cells as well those from specific glycoproteins, Thy1 and Ly5/T200, which exhibit differentiation related alterations. The highest priority of the study is the characterization of oligosaccharides containing polylactosamine structures because they have been recognized to be associated with differentiation and maturation in a number of biological systems, most notably the hematopoietic system. The cell types to be used in the current study will encompass T lymphoid and natural killer (NK) cells at various stages of differentiation and maturation. Cloned T and NK cells with different cytolytic or helper functions will also be studied. The biochemical characterization of cell surface glycoproteins will be accomplished using serial lectin chromatography, polylactosamine specific Datura stramonium and tomato lectins, and binding to anti-Ii affinity columns. Cell surface polylactosamine expression will be related to oligosaccharide alterations observed during ontogeny and maturation in the hematopoietic system. The role of oligosaccharides will be studied in anti-Thy1 induced T cell proliferation and NK-tumor cell adhesion and subsequent lysis. The identification of specific oligosaccharide alterations in discrete subsets of cells that have specific biological roles will be an important step in our understanding of differentiation, development and transformation.

这项研究的总体目标是定义生化改变 这发生在细胞表面糖蛋白的寡糖中 分化以及T淋巴细胞的功能表达和 天然杀手细胞。 N连接的寡糖的表达将是 从整个细胞中检查了特定糖蛋白Thy1的细胞。 和LY5/T200，它们表现出与分化相关的变化。 这 该研究的最高优先级是寡糖的表征 含有聚乳酰胺结构，因为它们已被识别为 与许多生物学的分化和成熟有关 系统，最著名的是造血系统。 要使用的单元格 在当前的研究中，将涵盖T淋巴机和天然杀手（NK） 分化和成熟的各个阶段的细胞。 克隆的T和NK 还将研究具有不同细胞溶液或辅助功能的细胞。 细胞表面糖蛋白的生化表征将是 使用串行凝集素色谱法，多乳糖胺特异性 datura stramonium和番茄凝集素，并与抗II亲和力结合 列。 细胞表面polactosamine的表达将与 在本体发育和成熟期间观察到的寡糖改变 造血系统。 将研究寡糖的作用 抗THY1诱导T细胞增殖和NK肿瘤细胞粘附和 随后的裂解。 特定寡糖的识别 具有特定生物学的细胞离散子集的改变 角色将是我们理解差异化的重要一步 发展和转变。