14TSB_SynBio A High Throughput Miniaturised Mass Spectrometry Tool for Profiling Synthetic Design Libraries

14TSB_SynBio 用于分析合成设计文库的高通量小型化质谱工具

• 批准号: BB/M005577/1
• 负责人: Thomas Ellis
• 金额: $ 11.87万
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2014
• 资助国家: 英国
• 起止时间: 2014 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FM005577%2F1
• 关键词: 14TSB_SynBio; Throughput; Miniaturised; Mass; Spectrometry

Over the last 10 years, the new field of synthetic biology has advanced the existing science of genetic modification by applying principles of engineering; design, simulation and testing. This approach has allowed us to predictably create exciting new technologies by modifying safe microbes with customised DNA to perform new tasks including computation, multi-input environmental sensing and efficient production of medicinal drugs and bioenergy, primarily through metabolic pathway engineering. As our ability to write DNA and engineer microbes accelerates, we are becoming limited by the lack of high-data, high-throughput cell measurement methods available with which to assess the performance of customised strains. The project links foundational synthetic biology and DNA assembly expertise at Imperial College London with microfluidics and mass spectrometry expertise at the industrial partner, Sphere Fluidics Limited, in order to build, test and demonstrate a novel microfluidics-mass spectrometry tool that should allow in-depth measurement of the performance of thousands of engineered microbes per run. The intention of the project is to develop an equipment set-up (interfaced microfluidics and mass spectrometry) and a methodology that together is a platform tool with which to characterise collections of thousands of engineered microbe strains. This tool can then be sold downstream as a service (or as an equipment set-up) for companies and institutions involved in synthetic biology.

在过去的十年中，合成生物学的新领域通过应用工程学原理来提高了遗传修饰的现有科学。设计，模拟和测试。这种方法使我们可以通过修改具有自定义DNA的安全微生物来执行新任务，包括计算，多输入环境感应和有效的药物药物和生物能源生产，主要是通过代谢途径工程来创造令人兴奋的新技术。随着我们编写DNA和工程师微生物加速的能力，我们受到缺乏高数据，高通量细胞测量方法的限制，可以评估自定义菌株的性能。该项目链接伦敦帝国学院的基础合成生物学和DNA组装专业知识，具有微流体和质谱专业知识，在工业合作伙伴Sphere Flicilics Limited中，以建立，测试和演示一种新型的微流体质谱学工具，应允许对数千个工程型微生物的绩效进行研究。该项目的目的是开发设备设置（接口微流体和质谱法），以及一种共同的方法，它是一种平台工具，可以表征成千上万的工程微生物菌株的集合。然后，该工具可以作为服务（或作为设备设置）在下游出售，用于参与合成生物学的公司和机构。