A High Throughput Miniaturised Mass Spectrometry Tool for Profiling Synthetic Design Libraries

用于分析合成设计文库的高通量小型化质谱工具

• 批准号: 101846
• 金额: $ 17.78万
• 依托单位: SPHERE FLUIDICS LIMITED
• 依托单位国家: 英国
• 项目类别: Collaborative R&D
• 财政年份: 2014
• 资助国家: 英国
• 起止时间: 2014 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=101846
• 关键词: Throughput; Miniaturised; Mass; Spectrometry; Tool

Synthetic biology has advanced genetic manipulation by applying engineering principles (Design-Build-Test) to enable new discoveries by modifying safe microbes with custom DNA. The ability to write DNA has accelerated, but synthetic biology is now limited by a lack of high-throughput measurement methods to assess performance of custom-built strains. The proposed project links foundational synthetic biology expertise at Imperial College London with microfluidics and mass spectrometry (MS) expertise at Sphere Fluidics Limited. This synergistic team will build, test and demonstrate a new microfluidics-MS platform that enables in-depth analysis of the performance of hundreds of thousands of engineered microbes per day (conventional techniques use high sample volumes and can only test around 10,000 samples a day). To date, most synthetic biologists have only been able to analyse one gene at a time. Uniquely, our MS approach will enable analysis of multiple genes and components of artificial pathways. This will accelerate the discovery, and improve production of new, valuable medicines and fuel sources, and generate valuable, nascent Tools and Services for commercialisation.

合成生物学通过应用工程原理（设计构建测试）通过使用自定义DNA修改安全的微生物来实现新发现，从而实现了遗传操作。编写DNA的能力已经加速了，但是合成生物学现在受到缺乏高通量测量方法来评估定制菌株的性能的限制。拟议的项目将伦敦帝国学院的基础综合生物学专业知识与Sphere Flingics Limited的微流体和质谱学（MS）专业知识联系起来。这个协同的团队将建立，测试和演示一个新的Microfluidics-MS平台，该平台可以深入分析每天成千上万工程的微生物的性能（常规技术使用较高的样品量，并且每天只能测试约10,000个样品）。迄今为止，大多数合成生物学家只能一次分析一个基因。独特的是，我们的MS方法将能够分析人工途径的多个基因和成分。这将加速发现，并改善新的，有价值的药物和燃料来源的生产，并为商业化生成宝贵的新生工具和服务。