Development Of Chemiluminometric μ-Flow-Through Detectors

化学发光μ流过式检测器的开发

• 批准号: 13555231
• 负责人: KIBA Nobutoshi
• 金额: $ 3.39万
• 依托单位: University of Yamanashi
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13555231/
• 关键词: FLOW SYSTEM; MICROFLUID SYSTEM; CHEMILUMINESCENCE; DETECTOR; MINIATURIZED; HYDROGEN PEROXIDE; HIGH THROUGHPUT; ENZYME; 化学発光検出; 検出器; 小型化; 過酸化水素; ハイスループット分析; 固定化酵素; マイクロTAS; 化学発光; 科学発光

A MICROMACHINED FLOW CELL (OVERALL SIZE; 25x25x1 mm^3) WAS DESIGNED FOR HIGH THROUGHPUT ANALYSIS. THE FLOW CELL CONSISTED OF A SILICON BOTTOM CHIP (0.5mm THICK) WHICH HAD A SPIRAL CHANNEL (20 TURNS), TWO PIERCED HOLES (1mm DIAMETER), AND A GLASS TOP PLATE (0.5mm THICK). THE CHANNEL (50cm LENGTH, 150μm WIDTH, 20μm DEPTH, 150μm SPACING BETWEEN CHANNELS) WAS PRODUCED BY ECHING. TW0 HOLES SERVE AS THE FLUID INLET (CENTER) AND OUTLET (SIDE). THE CHIP WAS PLACED IN FRONT OF A WINDOW OF A PHOTOMULTIPLIER TUBE. ALSO, TRANSPARENT FUSED SILICA CAPILLARY (50μm I. D., 375μm O. D.) WHICH IS COATED WITH TEFLON AF WAS USED FOR MORE RAPID ANALYSIS. THE CHIP AND CAPILLARY WERE USED AS FLOW CELLS IN A SINGLE-LINE CHEMILUMINOMETRIC FLOW INJECTION SYSTEM. PEROXIDASE (EC 1.11.1.7) WAS COVALENTLY IMMOBILIZED ON THE INNER SURFACE OF THE CHANNEL. THE SAMPLE VOLUME FOR ONE MEASUREMENT WAS 0.2μL. THE MAXIMUM SAMPLING RATE WAS 315/h AT A CARRIER SOLUTION FLOW RATE OF 10μl/min. A CALIBRATION GRAPH FOR H_20_2 WAS LINEAR FOR 5 nM -5μM. THE SYSTEM WITH μ-FLOW-THROUGH DETECTOR WAS APPLIED TO THE DETERMINATIONS OF H_20_2 IN ENVIROMENTAL SAMPLES AND OF QXIDASE SUBSTRATES IN BIOCHEMICAL SAMPLES.

设计用于高吞吐量分析的微机械流动池（总尺寸； 25x25x1 mm^3）。流动池由一个硅底部芯片（0.5mm厚）组成，该芯片具有螺旋通道（20圈），两个刺穿的孔（直径1mm）和一个玻璃顶板（0.5mm厚）。通过Eching产生通道（50厘米长，150μm宽度，20μm深度，150μm间距）。 TW0孔充当流体入口（中心）和出口（侧）。将芯片放在光电管管的窗户前。此外，使用透明的二氧化硅毛细管（50μmI。D.，375μmO。D.），该毛细管与Teflon AF涂有涂层进行更快的分析。芯片和毛细管在单线化学流量注射系统中用作流细胞。过氧化物酶（EC 1.11.1.7）在通道的内表面共价固定。一个测量的样品体积为0.2μl。在10μl/min的载体溶液流速下，最大采样率为315/h。 H_20_2的校准图为5 nm-5μm。将具有μ-Flow直通检测器的系统应用于在生化样品中的环境样品和Qxidase底物中H_20_2的测定。

项目成果

Kazue Tani: "Retention Behavior of Monosaccharides and Disaccharides on Titania."Chromatographia. 57. 409-412 (2003)

Kazue Tani：“单糖和二糖在二氧化钛上的保留行为。”色谱法。

Nobutoshi Kiba: "Flow-through Chemiluminescence Sensor Using Immobilized Oxidases for the Selective Determination of L-Glutamate in a Flow-Injection System."Analytical Sciences. 17. 929-933 (2001)

Nobutoshi Kiba：“使用固定化氧化酶的流通式化学发光传感器在流动注射系统中选择性测定 L-谷氨酸。”分析科学。

Nobutoshi Kiba: "Simultaneous Determination of Choline and Acetylcholine Based on a Trienzyme Chemiluminometric Biosensor in a Single Line Flow Injection System."Analytical Sciences. 19. 1647-1651 (2003)

Nobutoshi Kiba：“基于单线流动注射系统中的三酶化学发光生物传感器同时测定胆碱和乙酰胆碱。”分析科学。

Kazue Tani: "Evaluation of Titania as an Ion-Exchanger and as a Ligand-Exchanger in HPLC."Chromatographia. 55. 33-37 (2002)

Kazue Tani：“二氧化钛作为离子交换剂和配体交换剂在 HPLC 中的评估。”色谱法。

Nobutoshi Kiba: "Chemiluminometric Sensor for Simultaneous Determination of L-Glutamate and L-Lysine with Immobilized Oxidases in a Flow …"Analytical Chemistry. 74. 1269-1274 (2002)

Nobutoshi Kiba：“用固定化氧化酶在流动中同时测定 L-谷氨酸和 L-赖氨酸的化学发光传感器……”分析化学。74。1269-1274 (2002)