BIOCIDAL POLYMERS FOR VIRAL INACTIVATION IN PLASMA

用于灭活血浆病毒的杀菌聚合物

• 批准号: 2712400
• 负责人: JEFFREY F WILLIAMS
• 金额: $ 7.26万
• 依托单位: HALOSOURCE CORPORATION
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 1998-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2712400
• 关键词: Vesiculovirus; antiseptic sterilization; biomaterial development /preparation; biomaterial evaluation; blood proteins; coagulation factor IX; coagulation factor VIII; encephalomyocarditis virus; method development; polymers; protein structure function; serum; virus infection mechanism

Viral contamination is a constant threat in the production of plasma and derived therapeutic proteins (1,2). Currently, a variety of decontamination measures is available, including Pasteurization, solvent/detergent treatments and u/v irradiation; none provides assurance of efficacy against the range of viral pathogens that may be present (3). New methods are needed. Recent advances in N-Halamine polymer chemistry permit creation of contact biocidal surfaces, expressing rapid, potent efficacy against bacterial and viral pathogens in fluid streams (4,5,6). In this study, the feasibility of using these polymers is explored, for inactivating both enveloped and non-enveloped viruses. Functional retention of certain plasma proteins will also be determined. Plasma suspensions of enveloped and non-enveloped viruses (Vesicular stomatitis VSV and encephalomyocarditis EMCV) will be passed through columns of N- halamine polystyrene polymers, and flow rates and contact times varied to establish a) parameters necessary to bring about a 6 log inactivation, b) retention rates of coagulation factors F IX, F VIII as indicators of the practical potential of the process. Results will be used to determine the suitability of the method for extended studies on a wide range of viral targets and plasma protein functions, with additional polymers in the N-halamine series. PROPOSED COMMERCIAL APPLICATIONS: Novel biocidal polymers with rechargeable antimicrobial activity may prove effective for inactivation of viruses in plasma, plasma protein preparations, and therapeutic proteins made through transgenic animal technology. Commercial applications of this approach by fluid stream or batch treatment with polymers could be developed for manufacturers and blood product processors. Use of such products could have a serious impact on the safety of biological agents derived from human blood and animal blood or body fluids.

病毒污染是血浆生产和 衍生的治疗蛋白（1,2）。目前，各种各样 可以使用净化措施，包括巴氏灭菌， 溶剂/洗涤剂处理和U/V辐射；没有人提供保证 可能存在的病毒病原体范围的功效（3）。 需要新方法。 N-哈拉明聚合物化学的最新进展 允许创建接触式杀生物表面，表达快速，有效的 对流体流中细菌和病毒病原体的功效（4,5,6）。 在这项研究中，探索了使用这些聚合物的可行性，因为 灭活被包裹和非发育的病毒。功能 保留某些血浆蛋白也将确定。等离子体 被包裹和非发育病毒的悬浮液（囊泡气孔炎 VSV和脑膜炎EMCV）将通过N-的列。 Halamine聚苯乙烯聚合物，流速和接触时间各不相同 为了建立a）导致6 log失活所需的参数， b）凝血因子的保留率f ix，f viii作为指标 该过程的实际潜力。结果将用于确定 该方法适用于广泛研究的扩展研究 病毒靶标和血浆蛋白功能，并在 N-HALAMINE系列。 拟议的商业应用： 具有可充电抗菌活性的新型杀生物聚合物可能 证明对血浆，血浆蛋白的病毒失活有效 制剂和通过转基因动物制造的治疗蛋白 技术。通过流体流或 可以为制造商开发与聚合物的批处理处理 血产品处理器。使用此类产品可能会很严重 对源自人类血液的生物剂的安全性的影响 动物血液或体液。