DEVELOPMENT OF IN-SITSU MEMBRANE SEPARATION PROCESS FOR ORGANIC ACID FERMENTATION

有机酸发酵原位膜分离工艺的开发

• 批准号: 09650851
• 负责人: KONDO Kazuo
• 金额: $ 2.24万
• 依托单位: DOSHISHA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09650851/
• 关键词: Lactic Acid; Fermentation of Organic Acid; Cellulose Triacetate; Synergism; Microcapsule; 担体輸送

The aim of this research is to develop in situ membrane separation process for the fermentation of organic acid. Preparation of optically active lactic acid using fermentation has attracted much attention because the production of biodegarable poly(lactic acid) is commercialized. To reduce the level of end-product inhibition in the fermentation, many processes involving procedures to separate the products have been developed. Among the processes, solvent extraction with chemical reaction is promising from economical reason. Two critical problems in the lactic acid fermentation combined with the reactive extraction remains to be resolved. The first is the toxicity of organic solvents to microbes. To avoid the direct contact between the microbes and organic solvent, we prepared the extractant impregnated cellulose triacetate membrane or polystyrene-supported microcapsules. The permeation rate of lactic acid through the membrane was relatively fast, but the extractant leaked from the membrane or capsules and fermentation stopped because of the strong toxicity of the trace amount of extractant dissolved in the broth. The second problem is the difference in the optimum pH between fermentation and reactive extractions using less toxic extractants. To overcome this point, we developed the new synergistic extraction system of lactic acid using tri-n-octylamine and tri-n-butyphosphate. But mixed extractants could not applied to the membrane separation system was problem because cellulose triacetate was hydrolyzed by the alkaline solution used as a stripping reagent. We tried to prepare the microcapsules involving the mixed extractants. The attempt was successful and its possible to apply the microcapsules to the fermentation of lactic acid.

这项研究的目的是开发有机酸发酵的原位膜分离过程。使用发酵的光活性乳酸制备引起了很多关注，因为可生物凝聚聚（乳酸）的产生已商业化。为了降低发酵中最终产物抑制的水平，已经开发了许多涉及分离产品的程序的过程。在过程中，经济原因有望用化学反应提取溶剂。乳酸发酵中的两个关键问题与反应性提取尚有待解决。首先是有机溶剂对微生物的毒性。为了避免微生物与有机溶剂之间的直接接触，我们准备了浸渍的浸渍纤维素三乙酸膜或聚苯乙烯支撑的微胶囊。乳酸通过膜的渗透速率相对较快，但是由于溶解在肉汤中的痕量提取剂的较强毒性，提取剂从膜或胶囊中泄漏出来，发酵停止了。第二个问题是使用毒性较小的提取物在发酵和反应性提取物之间的最佳pH值差异。为了克服这一点，我们使用三 - 辛基胺和三丁基磷酸盐开发了新的乳酸协同提取系统。但是混合提取物无法应用于膜分离系统是问题，因为三纤维蛋白纤维素被用作剥离试剂的碱性溶液水解。我们试图准备涉及混合萃取剂的微胶囊。这种尝试是成功的，并且有可能将微胶囊应用于乳酸的发酵。

项目成果

近藤和生: "Fermentation of Lactic Acid Combined with Solvent Extraction of Membrane Separation Containing a Mobile Carrier"Recent Research DeveCopments in Fermentation& Bioengineering. 1. 241-252 (1998)

Kazuo Kondo：“乳酸发酵与含有移动载体的膜分离的溶剂萃取相结合”发酵与生物工程的最新研究进展 1. 241-252 (1998)。

Michiaki Matsumoto and Kazuo Knodo: "Fermentation of Lactic Acid Combined with Solvent Extraction of Membrane Separation Containing a Mobile Carrie"Recent Res. Develop.Ferment. Bioeng.. 1. 241-252 (1998)

Michiaki Matsumoto 和 Kazuo Knodo：“乳酸发酵与含有移动载体的膜分离溶剂萃取相结合”最近的研究。

M. Matsumoto: "Extraction Equilibria of Organic Acids with Tri-n-octylmethyl Ammonium Chloride"Solv. Extr. Res. Develop., Japan. 6. 144-150 (1999)

M. Matsumoto：“有机酸与三正辛基甲基氯化铵的萃取平衡”Solv。

Michiaki Matsumoto and Kazuo Kondo: "Synergistic Extraction of Organic Acids with Tri-n-Butyl phosphate and Tri-n-Octylamine"Proc. 3rd Taipei-Kyushu Joint Symposium on Chemical Engineering. 93-95 (1999)

Michiaki Matsumoto 和 Kazuo Kondo：“用磷酸三正丁酯和三正辛胺协同萃取有机酸”Proc。

松本 道明: "Permeation of Lactic Acid through Polymeric Membrane Containing Mobile Carriers" Proceedings of the 4th Sino-Japanese Liquid Membrane Symposium. 115-118 (1998)

Michiaki Matsumoto：“乳酸通过含有移动载体的聚合物膜的渗透”第四届中日液体膜研讨会论文集115-118（1998）。