Study on molecules which exhibit either inhibitory or stimulating activities to growth of pathogen, especially Vibribparahaemolyticus

对病原体特别是副溶血弧菌生长具有抑制或刺激活性的分子的研究

• 批准号: 18390135
• 负责人: HONDA Takeshi
• 金额: $ 10.77万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18390135/
• 关键词: Serum; growth factor; inhibitor; Vibiro narahaemolvticus; transferin; hemonexin

We found the activities which inhibit or stimulate the growth of bacteria in bovine serum and human serum as well. Inhibitory molecule for the bacterial growth (especially Vibrio parahaemolyticus) was purified by successive column chromatographies of Sephadex G75 and DEAE-Sephadex G25 and Mono Q (FPLC), and we obtained the molecules of either transferin or hemopexin or both. On the other hand, we are close to obtain highly purified molecule which exhibits stimulating activity to bacterial growth. The molecular weight seems to be less than 3000, and it is heat-stable (at 100℃). It contains Mg+ and Mo+. In conclusion, we found that there are still unidentified factors in sera which exhibit either inhibitory on stimulating activities on growth of pathogen.

我们在牛血清和人血清中发现了抑制或刺激细菌生长的活性，通过Sephadex G75和DEAE-Sephadex G25和Mono Q的连续柱层析纯化了细菌生长的抑制分子（特别是副溶血弧菌）。 FPLC），我们获得了转铁蛋白或血红素结合蛋白或两者的分子。另一方面，我们接近高度纯化的分子，该分子表现出刺激活性以获得细菌。分子量似乎小于3000，并且具有热稳定性（在100℃）。 总之，我们发现血清中仍然存在对刺激活性具有抑制作用的未知因子。关于病原体的生长。

项目成果

Identification of proteins secreted via Vibrio parahaemolyticus Typeffisecretion system 1.

副溶血性弧菌分泌系统分泌的蛋白质的鉴定1。

• 发表时间: 2006
• 期刊: Infect. Immun 74(2)
• 作者: Ono.;T.;K.S.;Park;M.;Ueta;T.;lida;T.;Honda
• 通讯作者: Honda

An experience of cholera outbreak in the North-Eastern region of Thailand.

泰国东北部地区爆发霍乱的经历。

• 发表时间: 2008
• 作者: Kazuhisa Okada;Siriporn Chantaroj;Amonrattana Roobthaisong;Takeshi Honda Pathom Sawanpanyalert.
• 通讯作者: Takeshi Honda Pathom Sawanpanyalert.

細菌性腸管感染症研究の現状

细菌性肠道感染研究现状

• 发表时间: 2007
• 作者: 韓賢子;蒲池一成;桑江朝臣;阿部章夫;荒川宜親;本田武司
• 通讯作者: 本田武司

A targeting approach for delivery of polymer microparticle-antibody conjugate against Vibrio parahaemolyticus-induced cytotoxicity to human intestinal epithelial cells.

一种针对副溶血性弧菌诱导的人肠上皮细胞细胞毒性的聚合物微粒-抗体缀合物的靶向递送方法。

• 发表时间: 2007
• 期刊: J. Drug Targeting 15(6)
• 作者: G Feng;T. Kodama;X. Chen;K. Okada;and T. Honda
• 通讯作者: and T. Honda

Pore formation of thermostable Vibrio parahaemolyticus in lipid bilayers.

脂质双层中热稳定副溶血弧菌的孔形成。

• 发表时间: 2006
• 期刊: Int J Toxicol 25(5)
• 作者: Takahashi A;Yamamoto C;Kodama T;Yamashita K;Harada N;Nakano M;Honda T;Nakaya Y
• 通讯作者: Nakaya Y