Study on the early process (especially adhesive and invasive process) of pathogenesis of bacterial enteropathogens and its cellular response

细菌性肠病菌发病的早期过程（特别是粘附和侵袭过程）及其细胞反应研究

基本信息

批准号：
14021055
负责人：
HONDA Takeshi
金额：
$ 38.4万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Priority Areas
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2005
项目状态：
已结题

项目摘要

(1) Result on enteropathogenic E. coli (EPEC, EHEC) : We found that cortactin and talin bind to Tir, an effector injected vir Type III secretion system (TTSS) and the binding is essential for causing attaching and effacing lesion formed by EPEC and EHEC. We also found that EspB forming tip of needle of TTSS also bind host molecule of a catenin which is essential for the A/E lesion formation.(2) Result on Vibrio parahaemolyticus : The most important virulence factor of this organism is TDH../TRH (an exotoxin), however, this may not be enough to full understand of pathogenesis. Whole genome analysis completed suggested us that this has two sets of TTSSs. TTSS-1 is present in large chromosome and secrete an effector having apoptotic cytotoxicity. On the other hand, TTSS-2 found in only clinical isolates is locate on small chromosome and unidentified effector which may have enteropathogenicity may be imvolved.(3) Result on Providencia alcalifaciens : We have found, for the first time, an outbreak attributed to P alcalifaciens (PA). We also found that this organism possess TTSS on a large plasmid.. As shown above and unlike our speculation that these 3 pathogens may have different mechanism for causing diseases, it became clear that the 3 pathogens possess a common process, TTSS, which may serve as one of the important virulence factor.

（1）结果是肠病大肠杆菌（EPEC，EHEC）：我们发现Cortactin和Talin与TIR结合，效应子注入了III型的III型分泌系统（TTSS），而结合对于引起EPEC和EHEC形成的附着和eff虫是必不可少的。我们还发现，ESPB形成TTS的针头还结合了链氨酸蛋白酶的宿主分子，这对于A/E病变的形成至关重要。（2）导致触发性的parahaemoloselicticus：该生物体最重要的毒力因子是TDH ../ TDH ../ TRH（exotoxin），但是，这可能是足够的，可以完全了解ptateneseasy ptatenesseason。整个基因组分析完成了，这表明我们有两组TTSS。 TTSS-1存在于大染色体中，并分泌具有凋亡细胞毒性的效应子。另一方面，仅在临床分离株中发现的TTSS-2位于可能具有肠病的小染色体和身份不明的效应子上。（3）结果是alcalifaciens的结果：我们首次建立的是，我们首次归因于p Alcalifaciens（Pa）。我们还发现，这种生物在大质粒上具有TTS。如上所述，与我们猜测这三种病原体可能具有不同的引起疾病的机制不同，很明显，这三种病原体具有常见的过程，TTSS，这可能是重要的毒力因子之一。