Study on the early process (especially adhesive and invasive process) of pathogenesis of bacterial enteropathogens and its cellular response

细菌性肠病菌发病的早期过程（特别是粘附和侵袭过程）及其细胞反应研究

基本信息

批准号：
14021055
负责人：
HONDA Takeshi
金额：
$ 38.4万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Priority Areas
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2005
项目状态：
已结题

项目摘要

(1) Result on enteropathogenic E. coli (EPEC, EHEC) : We found that cortactin and talin bind to Tir, an effector injected vir Type III secretion system (TTSS) and the binding is essential for causing attaching and effacing lesion formed by EPEC and EHEC. We also found that EspB forming tip of needle of TTSS also bind host molecule of a catenin which is essential for the A/E lesion formation.(2) Result on Vibrio parahaemolyticus : The most important virulence factor of this organism is TDH../TRH (an exotoxin), however, this may not be enough to full understand of pathogenesis. Whole genome analysis completed suggested us that this has two sets of TTSSs. TTSS-1 is present in large chromosome and secrete an effector having apoptotic cytotoxicity. On the other hand, TTSS-2 found in only clinical isolates is locate on small chromosome and unidentified effector which may have enteropathogenicity may be imvolved.(3) Result on Providencia alcalifaciens : We have found, for the first time, an outbreak attributed to P alcalifaciens (PA). We also found that this organism possess TTSS on a large plasmid.. As shown above and unlike our speculation that these 3 pathogens may have different mechanism for causing diseases, it became clear that the 3 pathogens possess a common process, TTSS, which may serve as one of the important virulence factor.

(1) 对肠致病性大肠杆菌 (EPEC, EHEC) 的结果：我们发现 cortactin 和 talin 与 Tir 结合，Tir 是一种注射 vir III 型分泌系统 (TTSS) 的效应子，并且这种结合对于引起 EPEC 形成的附着和消除病变至关重要和肠出血性大肠杆菌。我们还发现，形成 TTSS 针尖的 EspB 也与连环蛋白的宿主分子结合，连环蛋白对于 A/E 病变的形成至关重要。(2) 副溶血弧菌的结果：该生物体最重要的毒力因子是 TDH../然而，TRH（一种外毒素）可能不足以充分了解发病机制。完成的全基因组分析表明它有两组 TTSS。 TTSS-1存在于大染色体中并分泌具有凋亡细胞毒性的效应子。另一方面，仅在临床分离株中发现的TTSS-2位于小染色体上，并且可能涉及可能具有肠致病性的未识别效应子。(3)关于普罗威登斯菌的结果：我们首次发现归因于的爆发产碱杆菌（PA）。我们还发现该生物体在一个大质粒上具有 TTSS。如上所示，与我们推测这 3 种病原体可能具有不同的致病机制的推测不同，很明显，这 3 种病原体具有共同的过程 TTSS，这可能有助于为重要的毒力因子之一。