Molecular biochemical study on pathogenesis, especially toxin and adhesive factor, of Vibrio parahaemolyticus

副溶血性弧菌发病机制尤其是毒素和粘附因子的分子生化研究

• 批准号: 07407008
• 负责人: HONDA Takeshi
• 金额: $ 11.01万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07407008/
• 关键词: Thermostable direct hemolysin(TDH); TDH-related hemolysin (TRH); Vibrio parahaemolytiucs; Adhesion; 細胞浸入性; 定着因子; レセプター; リン酸化; 毒素; 変異毒素; サイトトキシン

The purpose of this research is to investigate detailed pathogenesis of Vibrio parahaemolyticus. Following results were obtained.1. Studies on thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH) : We found that TDH recognized a specific receptor through its N-teminal region and then induced phosphorylation of a 25 kDa protein, resulting in hemolysis. We also found that genes for TDH,TRH and urease are closely linked and present within 50kb-fragment (a pathogenicity island? ).2. Studies on adhesive factors : We identified cHA (cell-associated hemagglutinin) as an adhesive factor of V.parahaemolyticus. The cHA is a tetrameric protein consisting of 4 subunit of 26kDa and the receptor for cHA is a glycoconjugate having molecular weight of 17kDa.3. Other results : We also demonstrated the invasive character of V.parahaemolyticus in vitro cultured cell system, which may explain the cause of septicemia due to this organism. The methods detecting TDH,TRH or both were developed and evaluated, and a new variant of TDH was found.

这项研究的目的是研究颤动溶血的详细发病机理。获得以下结果。1。对热稳定直接血解素（TDH）和与TDH相关的血素蛋白（TRH）的研究：我们发现TDH通过其N激进区域识别特定的受体，然后诱导25 kDa蛋白的磷酸化，从而导致溶血。我们还发现，TDH，TRH和尿素酶的基因紧密相连，并存在于50KB碎片（致病岛？）.2。粘合因子的研究：我们将CHA（细胞相关的血凝素）鉴定为V.parahayticus的粘合剂。 CHA是一种四聚体蛋白，由4个亚基组成26KDA，CHA受体是一种糖缀合物，分子量为17KDA.3。其他结果：我们还证明了V.parahayticus在体外培养的细胞系统中的侵入性特征，这可能解释了由于这种生物的原因。检测和评估了检测TDH，TRH或两者的方法，并发现了TDH的新变体。

项目成果

本田 武司: "菌体外蛋白毒素の検出法" 日本臨床, 3 (1995)

Takeshi Honda：“细胞外蛋白质毒素的检测方法”日本临床，3（1995）

Suthienkul O., T.Iida, K.S.Park, M.Ishibashi, S.Supavej, K.Yamamoto and T.Honda: "Restriction fragment length polymorphism of the tdh and trh genes in clinical Vibrio parahaemolyticus strains." J.Clin.Microbiol.34 (5). 1293-1295 (1996)

Suthienkul O.、T.Iida、K.S.Park、M.Ishibashi、S.Supavej、K.Yamamoto 和 T.Honda：“临床副溶血性弧菌菌株中 tdh 和 trh 基因的限制性片段长度多态性。”

Nagamune,K.et al.: "Intramolecular chaperone activity of the pro-region of Vibrio cholerae El Tor cytolyisn." J.Biological Chem.272(2). 1338-1343 (1997)

Nagamune,K.et al.：“霍乱弧菌 El Tor 溶细胞作用前区的分子内伴侣活性。”

山本耕一郎他: "モダンメディア" 細菌性腸感染症に対するワクチン現状, 42(9):394-399 (1996)

Koichiro Yamamoto 等人：《现代媒体》细菌性肠道感染疫苗的现状，42(9):394-399 (1996)

本田武司、上田成子(共著): "「食中毒予防と対処のすべて」。" 法研, 212 (1997)

本田武 (Takeshi Honda) 和上田鸣子 (Naruko Ueda)（合著者）：“有关预防和处理食物中毒的一切。”