Research on enterohaemorrhagic E.coli (EHEC) including its epidemiology, pathogenesis, and development of diagnosis and treatment..

肠出血性大肠杆菌（EHEC）的研究，包括其流行病学、发病机制以及诊断和治疗的进展。

• 批准号: 08300012
• 负责人: HONDA Takeshi
• 金额: --
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08300012/
• 关键词: Escherichia coli; EHEC O157; Epidemiology; Pathogenesis; Diagnosis; Treatment

1.Epidemiology : Survey for EHEC in cattle, especially calf, was conducted in Okayama and Sizuoka prefectures and we found that about 0.1-1.0% of calf tested were positive for EHEC O157. The isolates were tested for biochemical character, antimicrobial sensitivity test, and molecular analyzes including PFGE.A questionnaire survey on EHEC infection was also conducted for clinicians around Osaka prefecture.2.Pathogenesis : Several animal models such as mouse, rat and rabbit were established. Those experiments suggested that VT showed direct toxicity to brain as well as renal tubular cells.3.Diagnosis : Molecular genetical techniques such as DNA-probe and PCR methods were developed for EHEC detection. Latex-oriented lactofferin (an indicator for neutrophil) assay using diarrheal stool was applied for quick suspicion of EHEC infection. Some detection kits for VT and EHEC O157 were also evaluated. Thrombomodulin in sera was revealed to be an indicator for early recongnition of HUS.4.Treatment : It was shown that anti-VT antiserum prevented mouse from lethality due to VT.Effect of anti-microbial agents on VT-production by EHEC was examined.

1.EpideMiology：在冈山和Sizuoka县进行了牛EHEC的调查，尤其是小牛的调查，我们发现，EHEC O157的小牛测约0.1-1.0％的测试是阳性的。测试了分离株的生化特征，抗菌敏感性测试以及包括PFGE在内的分子分析。还针对大阪县周围的临床医生进行了有关EHEC感染的问卷调查。这些实验表明，VT对脑和肾小管细胞表现出直接的毒性。3。诊断：开发了用于EHEC检测的分子遗传技术，例如DNA-探针和PCR方法。使用腹泻大便进行了面向乳胶的乳糖（一种嗜中性粒细胞指标）测定，以快速怀疑EHEC感染。还评估了VT和EHEC O157的一些检测试剂盒。血清中血管蛋白在血清中被发现是HUS早期重新处理的指标。4。处理：抗VT抗血清阻止了由于VT引起的小鼠对抗微生物剂对EHEC的VT产生效应的影响。

项目成果

Yamazaki, S., Z.Lin, H.Shirai, A.Terai, Y.Oku, H.Ito, M.Omura, T.Karasawa, T.Tsukamoto, H.Kurazono, and Y.Takeda.: "Typing of verotoxins by DNA colony hybridization with poly-and oligonucleotide probes, a bead-enzyme-linked immunosorbent assay, and polyme

Yamazaki, S., Z.Lin, H.Shirai, A.Terai, Y.Oku, H.Ito, M.Omura, T.Karasawa, T.Tsukamoto, H.Kurazono, and Y.Takeda.：“打字

五十嵐隆: "疫痢と腸管出血性大腸菌によるHUSとの関連" 日本医事新報. 3780. 113-114 (1996)

Takashi Igarashi：“流行性腹泻与肠出血性大肠杆菌引起的 HUS 之间的关系”日本医疗新报 3780. 113-114 (1996)。

Ssng, W. K.: "Haemorrhagic colitis due to Escherichia coli O157 : H7 in Kenya" J. Trop. Pediatrics. 42. 118-119 (1996)

Ssng, W. K.：“肯尼亚因大肠杆菌 O157 导致的出血性结肠炎：H7”J. Trop。

余 明順: "下痢原性大腸菌と赤痢菌の鑑別" Midical Technology. 24. 1281-1288 (1996)

于明顺：“致泻性大肠杆菌与志贺氏菌的鉴别”，医药技术，24。1281-1288（1996）。

吉田真一: "腸管出血性大腸菌O157感染症とその病態" ICUとCCU. 20. 817-826 (1996)

Shinichi Yoshida：“肠出血性大肠杆菌 O157 感染及其病理生理学”ICU 和 CCU。 20. 817-826 (1996)