Localization mechanism of a Ca^<2+> pump modulator, phospholamban in cardiac sarcoplasmic reticulum

Ca^2泵调节剂受磷蛋白在心脏肌浆网中的定位机制

• 批准号: 20790208
• 负责人: HONDA Takeshi
• 金额: $ 2.66万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20790208/
• 关键词: 化学架橋; 筋小胞体; 心不全; ホスホランバン; 心筋小胞体; 免疫沈降; アプタマー

Phospholamban (PLN) is a regulator of the myocardiac contractility in cardiac sarcoplasmic reticulum (SR). Controling the subcellular localization of PLN leads to the prospect for new therapy of heart failure. In this study, it was suggested that the C-terminal region of PLN is critical for localization of PLN to SR. Based on this finding, the identification of a putative protein interacting to the C-terminal region of PLN was attempted. Furthermore, a novel PLN-targeted aptamer was successfully developed as the useful molecular tool for detection of the PLN-binding protein.

Phospholamban（PLN）是心脏肌浆网（SR）中心肌收缩性的调节剂。控制PLN的亚细胞定位会导致对心力衰竭的新疗法的前景。在这项研究中，建议PLN的C末端区域对于将PLN定位到SR至关重要。基于这一发现，试图鉴定假定的蛋白质与PLN的C末端区域相互作用。此外，成功开发了一种新型的以PLN为目标的适体作为检测PLN结合蛋白的有用分子工具。

项目成果

In vitro selection and characterization of DNA aptamers specific for phospholamban

受磷蛋白特异性 DNA 适体的体外选择和表征

• 发表时间: 2009
• 期刊: Journal of Pharmacology and Experimental Therapeutics vol.229
• 作者: 田中貴絵;本田健;松浦健二;木村佳弘;乾誠
• 通讯作者: 乾誠