Development of regenerative therapy for jaw bone using scaffold with carbon nanotubes.

使用碳纳米管支架开发颌骨再生疗法。

• 批准号: 16390549
• 负责人: YOKOYAMA Atsuro
• 金额: $ 9.02万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16390549/
• 关键词: carbon nanotubes; scaffold; cell culture; osteoblast; bulk material; biocompatibilit; macrophage; osteogenesis; カーボンナノファイバー; 細胞形態; 表面処理; チタン; 骨髄細胞; DNAマイクロアレイ; GBR; 骨再生; 濾過膜

In this study, investigation of biocompatibility of carbon nanotubes (CNTs), cell culture on the scaffold using with CNTs and fabrication of bulk CNTs were carried out for the purpose of reconstruction of jaw bone using with CNTs.CNTs were implanted in the subcutaneous tissue in rats for 2 years, and histological and ultramicrostructural investigation to CNTs were done. Some CNTs were observed in lysosome in macrophages and only slight granulomatous inflammatory response was recognized around CNTs at 2 years, although granulation tissue with phagocytes was seen around CNTs at early stage after implant. The degree of aggregation of CNTs became to slight with time. Most of typical structure of CNTs like tubes was not changed after 2 years by observation using TEM. These results suggested that CNTs was not strong prophlogistic substance and structure of crystal of CNTs was stable in vivo.Scaffolds with CNTs were made by vacuum filtration of the dispersed CNTs slurry onto polycarbonate (PC) membrane. Saos-2 (human osteoblast-like cell) was cultured on the scaffolds. Proliferation and alkaline phosphatase activity of Saos-2 cultured on the CNTs scaffolds were higher than those of PC membrane. Application of the CNTs scaffold for guided bone regeneration membrane to bone defects made on rat parietal bone was effective in osteogenesis compared to PC membrane.Bulk CNTs materials were fabricated by spark plasma sintering (SPS). Physical and mechanical properties of bulk CNTs were close to those of bone, and biocompatibility was excellent. These results showed that bulk CNTs made by SPS method was suitable for bone substitute materials.From these results, it was suggested that regenerative therapy for jaw bone using with CNTs would be possible.

本研究以碳纳米管重建颌骨为目的，通过碳纳米管（CNT）的生物相容性研究、碳纳米管支架上的细胞培养以及块状碳纳米管的制备，将碳纳米管植入患者的皮下组织中。对大鼠进行了 2 年的实验，并对 CNT 进行了组织学和超微结构研究。在巨噬细胞的溶酶体中观察到一些碳纳米管，并且在植入后的早期阶段，碳纳米管周围发现了具有吞噬细胞的肉芽组织，但在2年时，碳纳米管周围仅发现了轻微的肉芽肿性炎症反应。随着时间的推移，碳纳米管的聚集程度变得轻微。通过使用 TEM 观察，大多数 CNT 管状结构在 2 年后没有发生变化。这些结果表明CNTs不是强引发剂物质，并且CNTs晶体结构在体内稳定。通过将分散的CNTs浆料真空过滤到聚碳酸酯(PC)膜上制备了CNTs支架。 Saos-2（人成骨细胞样细胞）在支架上培养。 CNTs支架上培养的Saos-2的增殖和碱性磷酸酶活性均高于PC膜。与PC膜相比，将碳纳米管支架用于引导骨再生膜应用于大鼠顶骨骨缺损，可有效促进成骨。通过放电等离子烧结（SPS）制备块状碳纳米管材料。大块碳纳米管的物理机械性能接近骨，且生物相容性优异。这些结果表明，通过SPS方法制备的块状碳纳米管适合作为骨替代材料。这些结果表明，利用碳纳米管对颌骨进行再生治疗是可能的。

项目成果

Biologocal behavior of hat-stacked carbon nanofibers in the subcutaneous tissue in rats.

帽子堆叠碳纳米纤维在大鼠皮下组织中的生物学行为。

• 发表时间: 2005
• 期刊: Nano Letters 5
• 作者: Yokoyama;A.;Sato;Y.;Nodasaka;Y.et al.
• 通讯作者: Y.et al.

カーボンナノチューブと金属内包カーボンナノカプセルの毒性評価

碳纳米管和金属包覆碳纳米胶囊的毒性评价

• 发表时间: 2006
• 期刊: 表面 44
• 作者: 佐藤義倫;宇尾基弘;横山敦郎;柴田健一郎;亘理文夫;田路和幸
• 通讯作者: 田路和幸

カーボンナノチューブの細胞内挙動

碳纳米管的细胞内行为

• 发表时间: 2006
• 期刊: バイオマテリアル-生体材料- 24
• 作者: Aoki;N.;Yokoyama;A.;Nodasaka;Y.et al.;横山敦郎
• 通讯作者: 横山敦郎

Carbon Nanotubes Deposited on Titanium Implant for Osteoblast Attachment

• DOI: 10.1166/jbns.2007.003
• 发表时间: 2007-06
• 期刊: Journal of Bionanoscience
• 作者: N. Aoki;A. Yokoyama;Y. Nodasaka;T. Akasaka;M. Uo;Y. Sato;K. Tohji;F. Watari

Cell Culture on a Carbon Nanotube Scaffold

• DOI: 10.1166/jbn.2005.048
• 发表时间: 2005-12-01
• 期刊: JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
• 影响因子: 2.9
• 作者: Aoki, Naofurni;Yokoyama, Atsuro;Watari, Furnio
• 通讯作者: Watari, Furnio