With the aim of expansion of the application of dental implants for the aged person and the patient who have systemic disease : the development of dental implants combined with the cell tranfected gene of bone related protein

以扩大种植牙在老年人及全身性疾病患者中的应用为目的：结合细胞转染骨相关蛋白基因的种植牙的开发。

基本信息

批准号：
13470407
负责人：
YOKOYAMA Atsuro
金额：
$ 8.13万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

项目摘要

The purpose of this study was expansion of the application of dental implants for sparse bone of the aged person and the patient who have systemic disease. Firstly, we investigate the adhesive potential of exogeneous osteopontin(OP) and osteonectin(ON) expressing osteoblastic cells that we have established. The expression of exogeneous OP and ON was confirmed by Western blotting. Parental Saos2, Saos2-puro, Saos2-pcDNA3, and Saos2-OP and Saos2-ON were seeded on the polystyrene dish at a density of 1.5 x 10^5 cells in serum-free DMEM. The cell adhesion rates(CAR) were counted after 6, 12 and 24 hours of incubation. Also, the cell adhesive shear force (F) and cell adhesive area (A) were measured in serum-free DMEM after 24 hours. The CAR of Saos2-OP was higher than those of parental cells (p<0.05), and that of Saos2-ON was lower than those of parental cells (p<0.05). F/A of Saos2-OP had higher than the parental cells, though no significance was observed in the difference between them. Th … More ese results suggest that human OP is a potent enhancer of osteoblast-like cell adhesion. Secondary, we investigated the effect of diabetes on bone tissue around endosseous implants after osseointegration. Titanium implants were inserted in the femora of rats. At 8 weeks after implantation, diabetes was induced by intra peritoneal injection of streptozotocin. Histopathological and histomorphometric evaluations were carried out. There was no effect of diabetes on the ratio of bone-implant contact, while the amount of newly formed bone in the control group was greater than that of the diabetic group. Next, we developed dental implants combined with the bone marrow cells. Undifferentiated bone marrow cells were cultured on dental implants with dexamethasone for 2 weeks. Dental implants with cultured bone marrow cells were implanted into subcutaneous tissue in rats. Histopathogical investigation and analysis by RT-PCR were done. At 4 weeks after surgery, bone tissue was observed on the surface of dental implants and expression of mRNA of osteocalcin was detected by RT-PCR. These results suggested that the dental implants combined with the cells transfected gene of bone protein were effective on the sparse bone of the aged person and the patient who have systemic disease. Less

本研究的目的是扩大牙种植体在老年人和患有全身性疾病的骨质疏松患者中的应用。首先，我们研究了表达骨桥蛋白（OP）和骨连接蛋白（ON）的成骨细胞的粘附潜力。我们已经通过蛋白质印迹证实了外源 OP 和 ON 的表达。以 1.5 x 10^5 个细胞的密度接种在无血清 DMEM 中的聚苯乙烯培养皿上，培养 6、12 和 24 小时后计算细胞粘附率 (CAR)。 24小时后在无血清DMEM中测量Saos2-OP的CAR高于亲代细胞。 (p<0.05)，Saos2-ON 的 F/A 低于亲本细胞 (p<0.05)，Saos2-OP 的 F/A 高于亲本细胞，但两者之间没有观察到显着性差异。这些结果表明，人类 OP 是成骨细胞样细胞粘附的有效增强剂。其次，我们研究了钛植入物后糖尿病对骨内植入物周围骨组织的影响。植入后8周，通过腹腔注射链脲佐菌素诱导糖尿病，并进行组织病理学和组织形态学评估，但糖尿病对骨植入物接触的比例没有影响。对照组新形成的骨量大于糖尿病组，接下来，我们开发了与骨髓细胞结合的牙种植体，并在牙种植体上培养了未分化的骨髓细胞。将培养有骨髓细胞的牙种植体植入大鼠皮下组织，并在术后4周进行组织病理学检查和RT-PCR分析，观察牙种植体表面的骨组织和表达。 RT-PCR检测骨钙素mRNA的含量，表明联合转染骨蛋白基因的细胞的牙种植体对于老年人和全身性疾病患者的骨质疏松有效。较少的