With the aim of expansion of the application of dental implants for the aged person and the patient who have systemic disease : the development of dental implants combined with the cell tranfected gene of bone related protein

以扩大种植牙在老年人及全身性疾病患者中的应用为目的：结合细胞转染骨相关蛋白基因的种植牙的开发。

基本信息

批准号：
13470407
负责人：
YOKOYAMA Atsuro
金额：
$ 8.13万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

项目摘要

The purpose of this study was expansion of the application of dental implants for sparse bone of the aged person and the patient who have systemic disease. Firstly, we investigate the adhesive potential of exogeneous osteopontin(OP) and osteonectin(ON) expressing osteoblastic cells that we have established. The expression of exogeneous OP and ON was confirmed by Western blotting. Parental Saos2, Saos2-puro, Saos2-pcDNA3, and Saos2-OP and Saos2-ON were seeded on the polystyrene dish at a density of 1.5 x 10^5 cells in serum-free DMEM. The cell adhesion rates(CAR) were counted after 6, 12 and 24 hours of incubation. Also, the cell adhesive shear force (F) and cell adhesive area (A) were measured in serum-free DMEM after 24 hours. The CAR of Saos2-OP was higher than those of parental cells (p<0.05), and that of Saos2-ON was lower than those of parental cells (p<0.05). F/A of Saos2-OP had higher than the parental cells, though no significance was observed in the difference between them. Th … More ese results suggest that human OP is a potent enhancer of osteoblast-like cell adhesion. Secondary, we investigated the effect of diabetes on bone tissue around endosseous implants after osseointegration. Titanium implants were inserted in the femora of rats. At 8 weeks after implantation, diabetes was induced by intra peritoneal injection of streptozotocin. Histopathological and histomorphometric evaluations were carried out. There was no effect of diabetes on the ratio of bone-implant contact, while the amount of newly formed bone in the control group was greater than that of the diabetic group. Next, we developed dental implants combined with the bone marrow cells. Undifferentiated bone marrow cells were cultured on dental implants with dexamethasone for 2 weeks. Dental implants with cultured bone marrow cells were implanted into subcutaneous tissue in rats. Histopathogical investigation and analysis by RT-PCR were done. At 4 weeks after surgery, bone tissue was observed on the surface of dental implants and expression of mRNA of osteocalcin was detected by RT-PCR. These results suggested that the dental implants combined with the cells transfected gene of bone protein were effective on the sparse bone of the aged person and the patient who have systemic disease. Less

这项研究的目的是扩展牙齿牙入的应用在老年人和患有全身性疾病的患者的稀疏骨骼中的应用。首先，我们研究了表达我们已经确定的骨细胞的外源骨桥蛋白（OP）和骨蛋白（ON）（ON）的粘合剂潜力。外源性OP的表达通过蛋白质印迹证实。将父母的Saos2，Saos2-Puro，Saos2-PCDNA3和SAOS2-OP和SAOS2-ON在聚苯乙烯盘中以1.5 x 10^5细胞的密度在无血清DMEM中的密度下播种。孵育6、12和24小时后计数细胞粘合率（CAR）。同样，在24小时后，在无血清DMEM中测量细胞粘附剪切力（F）和细胞粘附区（A）。 SAOS2-OP的汽车高于亲本细胞（P <0.05），SAOS2-ON的汽车低于亲本细胞的汽车（P <0.05）。 SAOS2-OP的F/A高于亲本细胞，尽管它们之间没有观察到显着性。 ……更多的结果表明，人类OP是成骨细胞样细胞粘合剂的潜在增强子。次要，我们研究了骨整合后糖尿病对骨组织周围骨组织的影响。将钛螺旋杆插入大鼠的股骨中。植入后8周，通过腹膜内注射链霉菌素诱导糖尿病。进行组织病理学和组织形态计量学评估。糖尿病对骨骼植入接触的比率没有影响，而对照组新形成的骨骼的量大于糖尿病组的骨骼。接下来，我们形成了牙齿刺，结合了骨髓细胞。将未分化的骨髓细胞培养在地塞米松的牙齿侵略性上2周。将具有培养的骨髓细胞的牙科植入物植入大鼠的皮下组织中。进行了RT-PCR的组织病毒投资和分析。手术后4周，在牙齿侵入的表面上观察到骨组织，并通过RT-PCR检测到骨钙素的mRNA表达。这些结果表明，与转染的骨蛋白转染基因相结合的牙齿朝向对老年人的稀疏骨骼和患有全身性疾病的患者有效。较少的