Real-time imaging analysis of synaptic plasticity underlying pheromonal memory.

信息素记忆背后的突触可塑性的实时成像分析。

基本信息

批准号：
17570056
负责人：
ICHIKAWA Masumi
金额：
$ 2.24万
依托单位：
Tokyo Metropolitan Organization for Medical Research
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

We have established a primary co-culture system for VN organ and AOB neurons to study the functional roles of the VN and AOB neurons in pheromonal signal processing and the synaptic plasticity. In the present project, we revealed that the maturation of VN neuron was induced by co-culture with the AOB neurons. It has been hypothesized that the differentiation and/or maturation of AOB neurons was modified by VN neurons via specific synaptic interactions between the sensory neurons and its target.In the present project, spine densities were quantitatively measured on culture time course in vitro. The densities of spines on AOB neurons were lower in co-culture than in single-culture. Synapse formation on spines of AOB neurons was analyzed immunocytochemically with anti-synaptophysin antibody. Ratio of density of synaptophysin-immunopositive spine / total spine density was larger in co-culture than in single-culture. The volume of spine-head was larger in co-culture than in single-culture. These changes were not recognized in co-culture condition without physical contacts between AOB neurons and VN neurons.We have performed real-time imaging of GFP transfected living AOB neurons in coculture with VN neurons. Rapid protrusion and retraction of filopodia and spine movement were observed. The difference in such motility and density of these protrusions was found according to the culture age and the neuron type. After 28 days in vitro, some new stable spines protruded from dendritic shaft directly, not grown up from filopodia were observed.The present results, thus, suggested that synapse formation on AOB neurons are modified by the contacts with VN neurons.

我们已经建立了一个用于VN器官和AOB神经元的主要共培养系统，以研究VN和AOB神经元在信息符号信号处理和突触可塑性中的功能作用。在本项目中，我们透露，VN神经元的成熟是与AOB神经元共培养的。据推测，AOB神经元的分化和/或成熟是通过VN神经元通过感觉神经元与其靶标之间的特定突触相互作用来修饰的。在本项目中，脊柱密度在体外培养时间过程中定量测量。 AOB神经元上的棘突密度在共培养中低于单一文化。用抗突触蛋白抗体分析了AOB神经元棘的突触形成。突触素 - 免疫阳性脊柱 /总脊柱密度的密度比单养殖中的密度大。脊柱头的体积在共培养中大于单一文化。这些变化在共培养条件下未被识别，而没有AOB神经元和VN神经元之间的身体接触。我们已经对与VN神经元共培养的GFP转染的活AOB神经元进行了实时成像。观察到丝状疾病和脊柱运动的快速突出和缩回。根据培养年龄和神经元类型，发现了这些突起的运动性和密度的差异。经过28天的体外，直接观察到从树突轴伸出的一些新的稳定棘突，未观察到从丝状疾病中长大的。因此，目前的结果表明，AOB神经元上的突触形成是通过与VN神经元的接触来修饰的。

项目成果

期刊论文数量（11）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Expression of a vomeronasal receptor gene (Vlr) and G protein alpha subunits in goats olfactory receptor neurons.

山羊嗅觉受体神经元中犁鼻受体基因 (Vlr) 和 G 蛋白 α 亚基的表达。

DOI：
发表时间：
2007
期刊：
Journal Comparative Neurology 503
影响因子：
0
作者：
Wakabayashi Y;Ohkura S;Okamura H;Mori Y;Ichikawa M
通讯作者：
Ichikawa M

Stable knock-down of vomeronasal receptopr genes in transgenic Xenopus tadpoles.

转基因非洲爪蟾蝌蚪中犁鼻受体基因的稳定敲低。

DOI：
发表时间：
2006
期刊：
Biochemical Biophysical Research Communication 345
影响因子：
0
作者：
Kashiwagi A;Kashiwagi K;Saito S;Date-Ito A;Ichikawa M;Mori Y;Hagino-Yamagishi
通讯作者：
Hagino-Yamagishi

Expression of a vomeronasal receptor gene (V1r) and G protein alpha subunits in goats olfactory receptor neurons.

山羊嗅觉受体神经元中犁鼻受体基因 (V1r) 和 G 蛋白 α 亚基的表达。