Morphological analysis of plastic changes induced by environment on synapses in rat olfactory system

环境引起的大鼠嗅觉系统突触塑性变化的形态学分析

基本信息

批准号：
06640871
负责人：
ICHIKAWA Masumi
金额：
$ 1.22万
依托单位：
Tokyo Metropolitan Institute for Neuroscience
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06640871/
关键词：
Oifaction Vomeronasal system Pheromon Synapse Plasticity ハムスター鋤鼻神経

项目摘要

It has been known that the soiled bedding and the urin contain pheromonal substances.Thus, in orderto study pheromonally induced synaptic plasticity in the vomeronasal system, effects of soiled bedding and urin has been examined in the accessory olfactory bulb (AOB).(1) The effects of exposure to soiled bedding on synaptic morphology in the AOB were examined in adult male rats.One group was exposed to bedding soiled by male and female rats (male and female group).Another group was exposed to bedding soiled by only males (male group).A third group was exposed to clean bedding (clean group).After 2 months, the mean size of the synapses was significantly greater in the male and female group than in the clean group, whereas that in the male group appeared to be intermediate between those in the male and female and the clean groups, but was not significantly different.These results suggest that exposure to a more complex soiled bedding environment can induce greater structural changes of the synapses in the AOB of male adult rats.(2) The effect of urinary pheromones on the synapses of male AOBs has been studied.Urine is collected every day from intact female hamsters, ovariectomized hamsters and male hamsters.Male hamsters are housed in isolation and exposed to one of four conditions : intact female urine (female group), urine of ovariectomized females (OVX group), male hamster urine (male group), and distillled water (water group).After being reared for 15 days, the synapse in the female group is significantly greaterr than that in the water and the male groups.This indicates that exposure to female pheromones induces morphological changes in the synaptic size in the male AOB.

众所周知，脏的被褥和尿液中含有信息素物质。因此，为了研究信息素诱导的犁鼻系统突触可塑性，我们研究了脏的被褥和尿液对副嗅球（AOB）的影响。（1）在成年雄性大鼠中检查暴露于弄脏的垫料对 AOB 突触形态的影响。一组暴露于雄性和雌性大鼠弄脏的垫料（男性和女性组）。另一组暴露于仅由男性弄脏的垫料（男性组）。第三组暴露于干净的垫料（清洁组）。2 个月后，突触的平均大小显着更大男性组和女性组均高于清洁组，而男性组似乎介于男性组、女性组和清洁组之间，但没有显着差异。这些结果表明，暴露于更复杂的污染被褥环境可以引起更大的结构变化(2)研究了尿液信息素对雄性AOB突触的影响。每天采集未绝育雌性仓鼠、去卵巢仓鼠和雄性仓鼠的尿液。雄性仓鼠隔离圈养，暴露于四种情况之一：完整的雌性尿液（雌性组）、切除卵巢的雌性尿液（OVX组）、雄性仓鼠尿液（雄性饲养15天后，雌性组的突触明显大于水组和雄性组。这表明暴露于雌性信息素引起了突触形态的变化。男性 AOB 的大小。