Mechanisms of synaptic plasticity at the single neuron level for understanding a pheromonal memory stored in the mouse accessory olfactory bulb.

单神经元水平的突触可塑性机制，用于理解小鼠副嗅球中存储的信息素记忆。

• 批准号: 23770075
• 负责人: MURATA Yoshihiro
• 金额: $ 2.33万
• 依托单位: Kochi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23770075/
• 关键词: 神経科学; 脳・神経; 生理学; 動物; 行動学; シナプス; 可塑性; 長期増強; 副嗅球; マウス; 神経科学; 脳・神経; 生理学; 動物; 行動学; シナプス; 可塑性; 長期増強; 副嗅球; マウス

In mice, some urinary pheromones of the unfamiliar male block pregnancy of the female, but those of the mating male do not. The inability of the mating male to disrupt the pregnancy depends on the memory of his pheromones formed by the female. The pheromonal memory is based on the neural changes in the accessory olfactory bulb (AOB), the first relay in the vomeronasal system. Microcircuits in the AOB include the prominent reciprocal dendrodendritic synapse between mitral cells, a single class of projection neurons, and granule cell interneurons. Long-term potentiation (LTP) at the mitral-to-granule cell synapse is expected to underlie the pheromonal memory. In this study I showed that antidromic stimulation of mitral cell axons induces long-term potentiation (LTP) at the mitral-to-granule cell synapse in slice preparations of the AOB, and that the late-phase LTP is protein synthesis-dependent. I also examined the effects of a protein kinase Mζ pseudosubstrate inhibitory peptide (ZIP), which inhibits LTP maintenance in the hippocampus and the neocortex, on the late phase of LTP at synapses in the mouse AOB. The results indicate that the maintenance of LTP at synapses in the AOB depends on ZIP-sensitive mechanisms.

在小鼠中，雌性陌生的雄性块怀孕的某些泌尿素，但交配雄性的尿素不会。交配男性无法破坏妊娠的能力取决于女性形成的信息素的记忆。信息符号记忆基于辅助嗅球（AOB）的神经变化，这是选民系统中的第一个继电器。 AOB中的微电路包括二尖瓣细胞，单一的投影神经元和颗粒细胞中神经元之间突出的互惠树突突触。二尖瓣到颗粒细胞突触的长期增强（LTP）预计将构成信息序列记忆的基础。在这项研究中，我表明，二尖瓣细胞轴突的抗体刺激在AOB的切片制剂中诱导长期增强（LTP），而后相LTP是蛋白质的合成依赖性的。我还检查了蛋白激酶Mζ假基底抑制性肽（ZIP）的影响，该肽抑制了海马和新皮层的LTP维持在小鼠AOB突触中LTP的晚期。结果表明，AOB中突触处的LTP的维护取决于邮政编码的机制。