Morphological analysis of synaptic plasticity underlying memory of pheromone

信息素记忆突触可塑性的形态学分析

基本信息

批准号：
15570058
负责人：
ICHIKAWA Masumi
金额：
$ 2.37万
依托单位：
Tokyo Metropolitan Organization for Medical Research
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15570058/
关键词：
Synapse Pheromon Plasticity Vomeronasal system Accessory olfactory bulb Co-culture Electron Microscope Immunocytochemistry 記憶

项目摘要

1.Morphological analysis on dynamics of functional molecules in synaptic plasticityLocalization of functional molecules on the synapse (receptor of glutamate, receptor of GABA, PSD 95) of accessory bulb was electron microscopically examined by immunocytochemically. Immunogold technique was best for study of distribution of functional molecules on postsynaptic density. It was revealed that many functional molecules were localized on the postsynaptic density.2.Morphological and pharmacological analysis of transmission from vomeronasal organ to accessory olfactory bulb.We have developed a primary co-culture system for VN organ and AOB to study functional roles of the VN and AOB neurons in pheromonal signal processing and the synaptic plasticity. In the present project, we revealed that the maturation of VN neuron was induced by co-culture with the AOB neurons. It has been hypothesized that the differentiation and /of maturation of AOB neurons was modified by VN neurons via specific synaptic interactions between the sensory neurons and its target.In the next project, Spine densities were quantitatively measured on culture time course in vitro (DIV). The densities of dendritic spines developmentaly changed in both the AOB single culture and co-culture with VN neurons. The densities of spines on AOB neurons were lower in co-culture than in single-culture. Synapse formation on spines of AOB neurons was analyzed immunocytochemically with anti-synaptophysin antibody. Ratio of density of synaptophysin-immunopositive spine / total spine density was larger in co-culture than in single-culture. The volume of spine-head was larger in co-culture than in single-culture. These changes were not recognized in co-culture condition without physical contacts between AOB neurons and VN neurons.The present results, thus, suggested that synapse formation on AOB neurons are modified by the contacts with VN neurons.

1.对功能分子在突触上（谷氨酸的受体，GABA的受体，PSD 95的受体，PSD 95）功能分子动力学的动力学分析是通过免疫细胞学上的电子显微镜检查的。免疫金技术最适合研究突触后密度的功能分子的分布。据表明，许多功能分子都定位在突触后密度上。2。从沃梅纳纳斯器官到辅助嗅球的传播的体形和药理分析。我们已经开发了VN器官的主要共培养系统，并研究了AOB研究的功能，以研究研究的功能在信息符号处理和突触可塑性中的VN和AOB神经元。在本项目中，我们透露，VN神经元的成熟是与AOB神经元共培养的。已经假设，VN神经元通过感觉神经元与其靶标之间的特定突触相互作用来修饰AOB神经元的分化和 / / / / / / / / /。。在AOB单培养物和VN神经元共培养中，树突状刺的密度也发生了变化。 AOB神经元上的棘突密度在共培养中低于单一文化。用抗突触蛋白抗体分析了AOB神经元棘的突触形成。突触素 - 免疫阳性脊柱 /总脊柱密度的密度比单养殖中的密度大。脊柱头的体积在共培养中大于单一文化。这些变化在共培养条件下未被识别，而没有AOB神经元和VN神经元之间的身体接触。因此，结果表明，AOB神经元上的突触形成是通过与VN神经元的接触来修饰的。