Morphological and Behavioral Study of Plasticity and Transplantation in the Brain

大脑可塑性和移植的形态和行为研究

基本信息

批准号：
63540604
负责人：
ICHIKAWA Masumi
金额：
$ 1.34万
依托单位：
Tokyo Metropolitan Institute for Neuroscience
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

项目摘要

Plasticity of neuronal connections is an important and interest field in neuroscience. We have studied the lesion-induced reorganization of neuronal connections. The aims of the present study were to clarify the relationship between functional recovery and reorganization of neuronal connections using behavioral study, to examine the reorganization in the intact brain using the technique of transplantation, and to study the factors which has been suggested to relate with the plasticity.(1) Behavioral study of the relationship between functional recovery and reorganization of neuronal connections.The lesion-induced reorganization of neuronal connections is one of the mechanism suspected of mediating recovery of function after injury to the adult CNS. Changes of olfactory behavior lesion in olfactory pathway was studied. The olfactory behavior recovered after damage. The time sequence of olfactory behavior is similar to the reorganization of neuronal connection following the lesion. This result indicates the relationship between functional recovery and reorganization.(2) Morphological study of synapse formation in transplanted neuron.We confirmed that the transplanted cells survived long term in the host brain. Thus, synapse formation of these transplanted cells was examined, but in order to examine the synapse formation, the neuronal processes of transplanted cells had to be labeled. This labeling technique is very difficult so that the synapse formation of transplanted neurons could not confirm yet. We continue to develop the labeling technique.(3) Developmental study of expression of oncogene and neuropeptide.The expression of oncogene (c-src) and neuropeptide (LHRH) in developing brain has been studied. Immunocytochemical study showed that c-src expressed in growth of dendrites and synapse formation and not in adult brain. This result indicates that c-src has an important role in development and also plasticity in brain.

神经元连接的可塑性是神经科学中一个重要且令人感兴趣的领域。我们研究了损伤引起的神经元连接重组。本研究的目的是通过行为研究阐明功能恢复和神经元连接重组之间的关系，利用移植技术检查完整大脑的重组，并研究与神经元连接重组相关的因素。 (1)功能恢复与神经元连接重组关系的行为学研究。损伤引起的神经元连接重组是疑似介导成人中枢神经系统损伤后功能恢复的机制之一。研究嗅觉通路中嗅觉行为病变的变化。嗅觉行为在受损后恢复。嗅觉行为的时间顺序类似于病变后神经元连接的重组。这一结果表明了功能恢复与重组之间的关系。(2)移植神经元突触形成的形态学研究。我们证实移植细胞在宿主大脑中长期存活。因此，检查了这些移植细胞的突触形成，但为了检查突触形成，必须标记移植细胞的神经元突起。这种标记技术非常困难，以至于移植神经元的突触形成还无法确定。我们继续开发标记技术。(3)癌基因和神经肽表达的发育研究。对癌基因(c-src)和神经肽(LHRH)在发育中的大脑中的表达进行了研究。免疫细胞化学研究表明，c-src 在树突生长和突触形成中表达，而不是在成人大脑中表达。这一结果表明c-src在大脑的发育和可塑性中具有重要作用。