Molecular analyzes of genetic variation produced by somatic cell hybridization for the use of plant breeding

用于植物育种的体细胞杂交产生的遗传变异的分子分析

基本信息

批准号：
04660002
负责人：
NIIZEKI Minoru
金额：
$ 1.34万
依托单位：
Hirosaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04660002/
关键词：
Oryza sativa L.Glycine max Medicago sativa Lotus corniculatus Somatic cell hybrid Nuclear DNA Mitochondrial DNA Chloroplast DNA oryza sativa イネマメ科植物細胞融合科間雑種属間雑種サザンブロット

项目摘要

Interfamilial and intergeneric somatic cell hybridization among a gramineous species (Oryza sativa L., strain A-58) and three leguminous species soybean (Glycine max (L.) Merr., cv.Harosoy), alfalfa (Medicago sativa L., cv.Dupuit) and birdsfoot trefoil (Lotus corniculatus L., cv.Viking) were examined. Both fragments of parent in some cases and altered mtDNAs were observed in the restriction profiles and Southern blots of 5 combinations of somatic hybrid calli. On the other hand, nuclear DNAs and cpDNAs were the same as those of one parent and they were identical with those of soybean, alfalfa or birdsfoot trefoil, for the banding patterns of restriction profiles and Southern blots in somatic hybrid calli. Therefore, it is assumed that some kinds of interaction between nucleus and cytoplasmic organelle and selective disadvantage for rice nucleus promote such a uniparental segregation of cpDNA.So far, it has been indicated that the organization of mitochondrial genome is correlative with morphological and physiological functions of plants. In this study, regenerated hybrid plants of soybean and birdsfoot trefoil, and of rice and birdsfoot trefoil, showed the morphological and physiologically abnormality. Actually, some regenerants from the hybrid calli of rice and birdfoot trefoil were tolerant of low temperature and low sunlight intensity in green house at wintertime. Thus, it is suggested that these regenerated hybrid plants presumably have the altered mtDNAs, as well as hybrids calli analyzed in this study.

禾本科物种（Oryza sativa L.，菌株 A-58）和三个豆科物种大豆（Glycine max (L.) Merr.，cv.Harosoy）、苜蓿（Medicago sativa L.，cv.Harosoy）之间的科间和属间体细胞杂交。对 Dupuit）和鸟足三叶草（Lotus corniculatus L.，cv.Viking）进行了检查。在 5 个体细胞杂种愈伤组织组合的限制性谱和 Southern 印迹中观察到了某些情况下的亲本片段和改变的 mtDNA。另一方面，在体细胞杂交愈伤组织中限制性谱和Southern印迹的条带模式方面，核DNA和cpDNA与亲本相同，并且与大豆、苜蓿或三叶草的核DNA和cpDNA相同。因此，推测细胞核和细胞质细胞器之间的某种相互作用以及水稻细胞核的选择性劣势促进了cpDNA的这种单亲分离。迄今为止，已经表明线粒体基因组的组织与水稻的形态和生理功能相关。植物。在本研究中，大豆与三叶草、水稻与三叶草的再生杂交植株表现出形态和生理异常。实际上，水稻和三叶草杂交愈伤组织的一些再生子能够耐受冬季温室中的低温和低光照强度。因此，表明这些再生的杂交植物可能具有改变的线粒体DNA，以及本研究中分析的杂交愈伤组织。