Study on mitotic and meiotic homologous recombination in plant and cultured cells of rice

水稻植株和培养细胞有丝分裂和减数分裂同源重组的研究

• 批准号: 15580001
• 负责人: NIIZEKI Minoru
• 金额: $ 2.37万
• 依托单位: Hirosaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15580001/
• 关键词: homologous recombination; RiLIM15; Alternative splicing; Meiosis; southern blot; Exon; Intron; gene family; 相同染色体組換え遺伝子; mRNA; cDNA; ソマクローナル変異; 培養細胞; OsRAD51; 相同染色体組換え; イネゲノム; モチ・ウルチ; 分離比; homologous recombination; RiLIM15; Alternative splicing; Meiosis; southern blot; Exon; Intron; gene family; 相同染色体組換え遺伝子; mRNA; cDNA; ソマクローナル変異; 培養細胞; OsRAD51; 相同染色体組換え; イネゲノム; モチ・ウルチ; 分離比

1.Analysis of occurrence of MCO in cultured cell of rice : Calli were induced from F_1 seed (Wxwx) of glutinous rice (wxwx) as a seed parent and non-glutious rice(WxWx) as a pollen parent. After regeneration of rice plamts, it was found a dominant homologous plant(WxWx) which might be segregated by the MCO.2.Analysis of structure and expression pattern of RiLIM15 in rice : In order to clarify the relationships between occurrence of MCO and expression of RiLIM15 gene in cultured cells and panicles investigated on the aspects of structure and expression of RiLIM15. Two RiLIM15 homologous RiLIM15A and RiLIM15B were isolated. In exon region, the DNA sequences for two genes were highly homologous but not intron regions. The amino acid sequences these two genes were highly homologous, sugggesting that they may be nearly equivalent in function. Analysis of cDNA sequences that eight patterns of deletions of DNA sequences in RiLIM15 cDNA clones derived from both meiotic and cultured cells. They may be resulted from alternative splicing. The putative mRNA with these deletion may translate different functions of proteins.3.Analysis of RAD51 in rice : RT-PCR was performed using total RNA extracted from the cultured cells, young meiotic panicles, mature leaves and X-ray treated seedlings. Expression of the RAD51 gene was found to occur in cultured cells, young meiotic panicles and X-ray treated seedlings, but not mature leaves. The gene possibly participates in DNA repariring of MCO.4.Application to substantial plant breeding : In nine generation of progenies regenerated from calli of cv.Akihikari it was observed that a line is 3-4 early heading date and almost the same number of panicle as the parent cultivar and significantly higher thousand kernel weight than the parent cultivar.

1.在水稻培养细胞中，MCO的发生分析是从粘性大米（WXWX）的F_1种子（WXWX）作为种子父母的，作为花粉父母作为粉米（WXWX）作为花粉父。大米plamts再生后，发现了一种主要的同源植物（WXWX），可能会通过MCO.2。RILIM15的结构和表达模式进行隔离：阐明MCO的发生与RILIM15基因在培养的细胞中的表达与结构和表达rilim15的表达的rilim15基因的表达之间的关系。分离了两个RILIM15同源性RILIM15A和RILIM15B。在外显子区域，两个基因的DNA序列是高度同源但内含子区域。这两个基因的氨基酸序列高度同源，建议它们在功能上几乎相当。分析RILIM15 cDNA克隆中DNA序列缺失的八种模式，这些cDNA克隆均来自减数分裂和培养细胞。它们可能是由替代剪接产生的。带有这些缺失的假定mRNA可能会翻译蛋白质的不同功能。3。RAD51在水稻：RT-PCR中的分析使用从培养的细胞，年轻的减数分裂圆柱，成熟的叶子和X射线治疗的幼苗中提取的总RNA进行。发现Rad51基因的表达发生在培养的细胞，年轻的减数分裂圆柱和经X射线处理的幼苗中，但没有成熟的叶子。该基因可能参与MCO.4的DNA。4。对大量植物育种的申请：在从CV.Akihikari的Calli中复制的九代后代中，观察到一条线是3-4个早期的早期日期，几乎相同的圆锥体数量是父母的质量，并且比附加质量较高。