Production of usefull agriculture traits by somatic cell hybrid of rice and leguminous plants

水稻和豆科植物体细胞杂交产生有用的农业性状

• 批准号: 07556001
• 负责人: NIIZEKI Minoru
• 金额: $ 4.16万
• 依托单位: Hirosaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07556001/
• 关键词: Rice; Soybean; Alfalfa; Birdsfoot trefoil; Asymmetric somatic hybrid; Low temperature and sunshine tolerance; Reciprocal translocation; Chromosome substitution; プロトプラスト; 細胞融合; アイソザイム; サザンブロット解析; タイズ

All combinations of the interfamilial and intergeneric somatic cell hybridizations between a gramineous species, rice and three leguminous species, soybean, alfalfa and birdsfoot trefoil were examined. The results are the following :1.Some regenerated plants from the hybrid calli of rice and birdsfoot trefoil displayd deep green leaves and vigorous growth under low sunlight intensity and low temperatures (20ﾟC) in a green house in the winter season.2.In the Southern blots analysis, the deletion of one rDNA fragment of birdsfoot trefoil and the addition of one fragment of alfalfa were observed in the somatic hybrid cell of birdsfoot trefoil and alfalfa. This result show that the intergeneric translocation may have occurred between the chromosomes of somatic hybrids.3.In the asymmetric somatic hybrids between alfalfa and birdsfoot trefoil, Southern blot analyzes of the chloroplast genomes indicated that either genome of both parents sorted out in all calli investigated.4.In the somatic cell hybrids between soybean and alfalfa, isozyme analyzes suggested that a single soybean chromosome was replaced with a alfalfa chromosome, while most of the chromosomes were those of soybean in the hybrid calli.5.In the somatic cell hybrids between rice and birdsfoot trefoil, a single plant obtained was birdsfoot trefoil type with numerous shoots and flowers. This plant type may be usable for the sloping land for controlling erosion and beautification.

对禾本科植物水稻与三种豆科植物大豆、苜蓿和三叶草之间的科间和属间体细胞杂交的所有组合进行了检测，结果如下： 1.水稻和三叶草杂交愈伤组织的再生植株。冬季在温室中低光照和低温（20°C）下，叶子深绿，生长旺盛。2.在南方印迹分析发现，在三叶草与苜蓿的体细胞杂交细胞中，发现了三叶草的一个rDNA片段的缺失和苜蓿的一个片段的添加，这表明体细胞杂交体的染色体之间可能发生了属间易位。 3.在苜蓿和三叶草的不对称体细胞杂交中，叶绿体基因组的Southern印迹遗传学表明，双亲的基因组全部排序4.在大豆和苜蓿的体细胞杂交中，同工酶谱系表明单个大豆染色体被苜蓿染色体取代，而杂交愈伤组织中的大部分染色体是大豆的染色体。5.在体细胞中水稻和三叶草的杂交，获得的单株是具有大量芽和花的三叶草类型，该植物类型可用于坡地种植。控制侵蚀和美化。

项目成果

Nakajo, S.: "Nuclear and organelle behavior of Lotus corniculatus L.in somatic cell hybrid callus." Lotus Newslett.26. 9-11 (1996)

Nakajo, S.：“百脉根在体细胞杂交愈伤组织中的核和细胞器行为。”

Nakajo, S.: "Organelle DNA analysis of somatic cell hybrid between gramineus and leguminous species" Bull.Fac.Agric.Hirosaki Univ.58. 22-33 (1995)

Nakajo, S.：“禾本科和豆科物种之间体细胞杂交的细胞器 DNA 分析”Bull.Fac.Agric.Hirosaki Univ.58。

Niizeki,M.: "Science of the Rice Plant.Vol.3.Genetics" T.Matsuo et al.(eds) Food and Agricultue Policy Research Center,Tokyo, 1003 (1997)

Niizeki,M.：“水稻科学.Vol.3.遗传学”T.Matsuo 等人（编）食品和农业政策研究中心，东京，1003（1997 年）