Production of usefull agriculture traits by somatic cell hybrid of rice and leguminous plants

水稻和豆科植物体细胞杂交产生有用的农业性状

• 批准号: 07556001
• 负责人: NIIZEKI Minoru
• 金额: $ 4.16万
• 依托单位: Hirosaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07556001/
• 关键词: Rice; Soybean; Alfalfa; Birdsfoot trefoil; Asymmetric somatic hybrid; Low temperature and sunshine tolerance; Reciprocal translocation; Chromosome substitution; プロトプラスト; 細胞融合; アイソザイム; サザンブロット解析; タイズ; Rice; Soybean; Alfalfa; Birdsfoot trefoil; Asymmetric somatic hybrid; Low temperature and sunshine tolerance; Reciprocal translocation; Chromosome substitution; プロトプラスト; 細胞融合; アイソザイム; サザンブロット解析; タイズ

All combinations of the interfamilial and intergeneric somatic cell hybridizations between a gramineous species, rice and three leguminous species, soybean, alfalfa and birdsfoot trefoil were examined. The results are the following :1.Some regenerated plants from the hybrid calli of rice and birdsfoot trefoil displayd deep green leaves and vigorous growth under low sunlight intensity and low temperatures (20ﾟC) in a green house in the winter season.2.In the Southern blots analysis, the deletion of one rDNA fragment of birdsfoot trefoil and the addition of one fragment of alfalfa were observed in the somatic hybrid cell of birdsfoot trefoil and alfalfa. This result show that the intergeneric translocation may have occurred between the chromosomes of somatic hybrids.3.In the asymmetric somatic hybrids between alfalfa and birdsfoot trefoil, Southern blot analyzes of the chloroplast genomes indicated that either genome of both parents sorted out in all calli investigated.4.In the somatic cell hybrids between soybean and alfalfa, isozyme analyzes suggested that a single soybean chromosome was replaced with a alfalfa chromosome, while most of the chromosomes were those of soybean in the hybrid calli.5.In the somatic cell hybrids between rice and birdsfoot trefoil, a single plant obtained was birdsfoot trefoil type with numerous shoots and flowers. This plant type may be usable for the sloping land for controlling erosion and beautification.

检查了格拉分离物，大米和三种豆类物种，大豆，苜蓿和鸟鸟夫特叶状物质之间家庭间和基因间体细胞杂交的所有组合。 The results are the following :1.Some regenerated plants from the hybrid calli of rice and birdsfoot trefoil displayed deep green leaves and vigorous growth under low sunlight intensity and low temperatures (20ﾟC) in a green house in the winter season.2.In the Southern blots analysis, the deletion of one rDNA fragment of birdsfoot trefoil and the addition of one fragment of alfalfa were observed in the somatic hybrid cell of birdsfoot三叶草和苜蓿。该结果表明，基因间易位可能发生在体细胞杂交染色体之间。3。在苜蓿和鸟蝇之间的不对称体细胞杂交中，对叶绿体基因组的Southern印迹分析表明，在所有calli calli中，所有calli calli的基因组都分析了所有calli。建议将单个大豆染色体替换为苜蓿染色体，而大多数染色体是杂种calli中的大豆的染色体。5。在水稻和鸟鸟脚三叶草之间的体细胞混合体中，获得的单个工厂是鸟类fort虫，是鸟类小脚型，带有许多芽和花朵。这种植物类型可能可用于控制侵蚀和美丽的倾斜土地。