Purchase of a Flow Cytometer for the Large-scale Assessment of Biogenic Particles in Estuarine and Coastal Systems

购买流式细胞仪用于大规模评估河口和沿海系统中的生物颗粒

• 批准号: 9713924
• 负责人: Thomas Malone
• 金额: $ 10.44万
• 依托单位: University of Maryland Center for Environmental Sciences
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-01-01 至 1998-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9713924&HistoricalAwards=false
• 关键词: Purchase; Flow; Cytometer; Large; scale

This project will provide for the acquisition of a standard bench top flow cytometer with argon laser illumination, five parameter analysis, and cell sorting capability. Flow cytometry is an important tool for interdisciplinary research on the dynamics of microbial populations and biogenic particles that participate in the cycling and flux of major elements in aquatic ecosystems of the land-sea margin. Flow cytometry was originally developed as a tool to analyze individual cells or biological particles in cellular biology and clinical research. It has more recently found wide applications in aquatic biology, because it can provide a level of temporal and spatial resolution of the abundance and characteristics of biogenic and non-biological particles in aquatic systems that no other current technology can match. This level of resolution is currently needed to complement largescale models of elements cycling in estuaries and oceans, where the distribution and characteristics of biological particles are the key factors that determine the fats and flux of essential chemical components. Thus, flow cytometry is no longer a technology restricted to clinical studies, but can profitably complement other techniques routinely used in aquatic ecology and biochemistry. This proposal represents an attempts to expand the use of flow cytometry in the study of the density, distribution, cellular characteristics, taxomic composition and trophic interactions of biogenic particles in estuaries and continental shelf ecosystems of the middle Atlantic region. The instrument will reside at the Horn Point Environmental Laboratory which has made a major commitment to the study of plankton dynamics, physical oceanography, and physical-biological interactions in estuarine and marine ecosystems. The instrument is extremely versatile and will be used in laboratory-and ship-based studies as well as in laboratory course work. Operation is relatively easy and user-friendly, makin g it an ideal tool not only for ecological research, but also for education.

该项目将购买一台标准台式流式细胞仪，具有氩激光照明、五参数分析和细胞分选功能。 流式细胞术是跨学科研究参与陆海边缘水生生态系统主要元素循环和流动的微生物种群和生物颗粒动态的重要工具。 流式细胞术最初是作为细胞生物学和临床研究中分析单个细胞或生物颗粒的工具而开发的。 最近，它在水生生物学中得到了广泛的应用，因为它可以提供水生系统中生物和非生物颗粒的丰度和特征的时间和空间分辨率，这是当前其他技术无法比拟的。 目前需要这种水平的分辨率来补充河口和海洋中元素循环的大规模模型，其中生物颗粒的分布和特征是决定必需化学成分的脂肪和通量的关键因素。 因此，流式细胞术不再是仅限于临床研究的技术，而是可以有利地补充水生生态和生物化学中常规使用的其他技术。该提案代表了扩大流式细胞术在中大西洋地区河口和大陆架生态系统中生物颗粒的密度、分布、细胞特征、分类组成和营养相互作用研究中的应用的尝试。 该仪器将驻留在霍恩波因特环境实验室，该实验室主要致力于研究浮游生物动力学、物理海洋学以及河口和海洋生态系统中的物理-生物相互作用。 该仪器用途极其广泛，可用于实验室和船基研究以及实验室课程工作。 操作相对简单且用户友好，使其成为生态研究和教育的理想工具。