Laser Scanning Cytometer for Purdue University

普渡大学激光扫描细胞仪

• 批准号: 6876911
• 负责人: JOSEPH PAUL ROBINSON
• 金额: $ 34.2万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-01 至 2006-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6876911
• 关键词: biomedical equipment purchase; biomedical facility; biomedical resource; flow cytometry; health science research support; molecular /cellular imaging

DESCRIPTION (provided by applicant): Flow cytometry capabilities have been maturing at Purdue University over the past 15 years. Since 1987, we have upgraded our flow cytometers and imaging instruments including support from SIG (1993, 2000, 2001) which allowed us to maintain high quality services and instruments to the faculty of the university. Since 1988, the Purdue University Cytometry Laboratories (PUCL) has maintained continuous service to the faculty. Over 366 faculty have used the facility in the past 15 years. Over 300 graduate students have been trained at advanced levels. The PUCL facility however, is not just about providing instrument support to researchers. Once the instruments are in place, we provide excellent technical support and quality advice. We maintain 4 full time technicians and a software expert whose entire role is to provide quality support to investigators. Instrumentation that comes into this facility is guaranteed to be not only available to faculty, but is provided a la carte with a commitment to quality service. We successfully introduced flow cytometry to many faculty who were not aware of its capability and supported those who have driven our program by participating in our SIG grants. We now find ourselves challenged by the significant development of cytomics - functional analysis with a higher need for parameterized morphology and sub-cellular localization and gene expression on cells in cell cultures and in tissue while maintaining spatial correlations. The Compucyte iCyte Imaging Cytometer is a commercially available cytometer that offers this capability for which this application requests funding. Although driven by 4 primary and 6 secondary users, our track record predicts that it will be used by many more faculty as our experience in its operation and management matures. Placement of a shared resource into a facility that has a strong track record of sharing and support is a cost effective way of supporting NIH funded researchers at this institution.

描述（由申请人提供）：在过去的15年中，普渡大学的流式细胞仪能力一直在成熟。自1987年以来，我们升级了流式细胞仪和成像工具，包括SIG（1993，2000，2001）的支持，使我们能够将高质量的服务和工具维持到大学学院。自1988年以来，普渡大学的细胞仪实验室（PUCL）一直为教师提供持续的服务。在过去的15年中，超过366位教职员工使用了该设施。超过300名研究生接受了高级培训。但是，PUCL设施不仅是为研究人员提供工具支持。仪器到位后，我们提供出色的技术支持和质量建议。我们维护4位全职技术人员和一名软件专家，其整个职责是为调查人员提供质量支持。保证进入该设施的仪器不仅可供教职员工使用，而且还提供了对优质服务的承诺。我们成功地将流式细胞仪引入了许多教师，他们不知道其能力，并通过参与我们的SIG赠款来支持那些驱动我们计划的人。现在，我们发现自己受到细胞学学的重大发展的挑战 - 功能分析，对细胞培养和组织中细胞中细胞的参数形态和亚细胞定位以及基因表达的需求更高，同时保持空间相关性。 Compucyte Icyte成像细胞仪是一种商业上可用的细胞图仪，可提供此应用程序请求资金的能力。尽管由4个主要和6个二级用户驱动，但我们的往绩记录预测，它将被许多教师用作我们的运营和管理成熟的经验。将共享资源的放置在具有共享和支持方面有很强记录的设施中，这是支持该机构的NIH资助研究人员的一种经济有效方法。