Equipment Core

装备核心

• 批准号: 6893254
• 负责人: RICHARD BRIAN DYER
• 金额: $ 17.82万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6893254
• 关键词: biomedical equipment purchase; biomedical equipment resource; biomedical facility; fluorescence spectrometry; infrared spectrometry; lasers; relaxation spectrometry; ultraviolet spectrometry

Core B - 'The Equipment Core'. The 'Equipment Core' supports the major equipment used to characterized the dynamical nature of the protein systems of the various projects of the Program Project. Two spectroscopic approaches are supported: 1. Temperature-jump relaxation spectroscopy. Three instruments are employed to cover the entire time range from 10 ps to minutes (or longer) in three overlapping time domains. (a) 10 ps - 10 ns. The T-jump is performed by heating water by laser irradiation, and the resolution of the instrument is set by the thermalization time of the absorbed energy. This picosecond instrument is located at Los Alamos. Both IR and UV/Vis absorbance and emission can be performed as probes of protein structure. (b) 15 ns-10 ms. The T-jump is achieved by heating water by laser irradiation. Three separate spectrometers will be employed. Two instruments are located at Albert Einstein and perform UV/Vis absorbance and/or fluorescence. IR transient studies will be performed at Los Alamos. (c) 1 ms - minutes (or longer). This instrument will be purchased and based at Einstein. The T-jump is achieved by mixing two solutions at different temperatures and holding the mixed solution at a final temp. Optical (UV/Vis) emission and or absorbance and IR absorbance spectroscopies are employed as structural probes. 2. A static isotope editing IR difference spectrometer located at Albert Einstein. In general, static thermal measurements precede T-jump kinetic studies. These calibrate the expected signal size to be observed in the kinetic work and also sets the magnitude of signal at infinite time in the kinetics experiments.

核心 B -“设备核心”。 “设备核心”支持用于表征该计划项目各个项目的蛋白质系统动力学性质的主要设备。支持两种光谱方法： 1. 温跃弛豫光谱。采用三种仪器覆盖三个重叠时域中从 10 ps 到分钟（或更长时间）的整个时间范围。 (a) 10 皮秒 - 10 纳秒。 T-jump是通过激光照射加热水来进行的，仪器的分辨率由吸收能量的热化时间设定。该皮秒仪器位于洛斯阿拉莫斯。红外和紫外/可见吸收和发射都可以作为蛋白质结构的探针。 (b) 15 纳秒-10 毫秒。 T-jump是通过激光照射加热水来实现的。三个分开 将使用光谱仪。阿尔伯特·爱因斯坦有两台仪器，用于执行紫外/可见光吸收和/或荧光分析。红外瞬态研究将在洛斯阿拉莫斯进行。 (c) 1 毫秒 - 分钟（或更长）。该仪器将在爱因斯坦购买并安装。 T-jump 是通过在不同温度下混合两种溶液并将混合溶液保持在最终温度来实现的。光学（紫外/可见）发射和/或吸光度和红外吸光度光谱被用作结构探针。 2. 位于阿尔伯特·爱因斯坦的静态同位素编辑红外差异光谱仪。一般来说，静态热测量先于 T 跳跃动力学研究。这些校准了动力学工作中要观察到的预期信号大小，并设置了动力学实验中无限时间的信号幅度。