MULTIPHOTON MICROSCOPE FOR PURDUE UNIVERSITY

普渡大学多光子显微镜

• 批准号: 6291360
• 负责人: JOSEPH PAUL ROBINSON
• 金额: $ 50万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-05-01 至 2003-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6291360
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; microscopy

This application for a multiphoton microscope builds upon the past six years of developing confocal microscopy applications in the Cancer Center and across the Purdue University campus. There are currently no multiphoton microscopes at this university. Over the past few years, a steady number of requests to perform experiments that exceeded the current confocal capability, required us to consider a more advanced technology. Two specific areas of experimentation created significant difficulties for our current confocal microscope system: deep tissue imaging and analysis of live cell systems in a kinetic protocol. The deep tissue imaging became a recurrent theme of the requests to use the confocal and culminated in the conclusion that we had reached the abilities of our current instrument. Our current instrument is still a very important instrument. Indeed, it has been a tremendous resource and we anticipate a continuation of the use of this instrument. Specifically it has become the instrument of choice for reflection imaging and will continue to play a significant role in the campus's biological imaging program. The second area of difficulty faced by our confocal users was the long term imaging of live cells. These assays include functional assays, observations of dividing or differentiation cells, phenotyping deep tissue cells, and functional analysis of cells including calcium flux. Live cell studies are in strong demand and will be difficult to perform adequately with our present system. The instrument requested will bring to Purdue University a capability it does not presently possess. We have waited for nearly 3 years, since originally considering our need for multiphoton. While we had demonstrated uses three years ago, it was our opinion that the technology was then too immature. Present-day almost turn-key systems are more desirable in our environment. Funding of this application will bring a significant advance to our present confocal capabilities.

该应用多光子显微镜的应用是在过去六年的癌症中心和普渡大学校园中开发共聚焦显微镜应用的基础上。该大学目前没有多光子显微镜。在过去的几年中，执行超过当前共聚焦功能的实验的稳定要求，要求我们考虑更先进的技术。实验的两个特定领域给我们当前的共聚焦显微镜系统带来了重大困难：深层组织成像和动力学方案中活细胞系统的分析。深层组织成像成为使用共焦和最终达到的要求的重复主题，这是我们已经达到了当前乐器的能力的结论。我们目前的仪器仍然是非常重要的工具。的确，这是一个巨大的资源，我们预计该工具的使用会延续。具体而言，它已成为反射成像的首选工具，并将继续在校园的生物成像程序中发挥重要作用。我们的共聚焦使用者面临的难度的第二个领域是活细胞的长期成像。这些测定包括功能测定，分裂或分化细胞的观察结果，表型深层组织细胞以及包括钙通量在内的细胞的功能分析。实时细胞研究的需求很强，我们目前的系统将很难充分执行。要求的仪器将带给普渡大学它目前所没有的能力。我们已经等待了将近3年，因为最初考虑了我们对多光子的需求。尽管我们三年前证明了使用的使用，但我们认为这项技术太不成熟了。在我们的环境中，当今几乎是键的系统更为理想。该申请的资金将为我们目前的共聚焦能力带来重大进步。