Purchase of a New confocal Zeiss LSM 510 v3.2

购买新的共焦蔡司 LSM 510 v3.2

• 批准号: 6878170
• 负责人: Vickery E Trinkaus-Randall
• 金额: $ 42.55万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6878170
• 关键词: biochemistry; bioimaging /biomedical imaging; biomedical equipment purchase; confocal scanning microscopy; eye; molecular /cellular imaging

DESCRIPTION (provided by applicant): The overall goal of this resubmission is to increase the research capabilities of our current confocal facility with the purchase of a new Zeiss inverted LSM 510 v3.2. The existing confocal (LSM 510 v1.6) was purchased in 1998 and is currently the only confocal facility on the medical campus. While it is used mainly by the major user group it is made available to the entire Boston University Medical School Community and is used to or over capacity and is aging. Our goal is to purchase a new confocal microscope that will expand the facility and allow for new technologies available to all investigators. The v1.6 will be maintained and will serve as a teaching instrument, be available for users with conventional needs and for overflow needs. As we are currently above capacity an upgrade would not benefit additional members and allow for a larger user base for the university. We are requesting a v3.2 LSM 510 mounted on an Axiovert 200M with the LSM #18 405/AR/Dual HENE/3 PMT and 4 internal detector channels. We are also requesting a motorized stage to support a large number of live and fixed studies that are currently performed. The Physiology, multi-time series and 3D analysis software are also critical to support the investigators needs as they have evolved and include Ca2+ imaging with several trigger points. The list also includes investigators who have approached me but can not use the current instrument as it does not support their experimentation needs. Extensive data has been collected on the existing microscope and users are now being constrained by the limitations of the technology and the fact that it is used to capacity. The major users routinely perform live cell imaging experiments over time to assess development or follow the response to injury. The Dean of the Medical School supports this instrument as the facility for the University and in accordance with that supports a full time person to run the confocal on a daily basis. Dean McCahan also supports the service contracts and the faculties that are needed to support the confocal systems and work station.

描述（由申请人提供）：此重新提交的总体目标是通过购买新的Zeiss倒置LSM 510 v3.2来提高我们当前共聚焦设施的研究能力。现有的共聚焦（LSM 510 V1.6）于1998年购买，目前是医疗校园中唯一的共聚焦设施。虽然主要用户组主要使用它，但可用于整个波士顿大学医学院社区，并习惯了或过度衰老。我们的目标是购买一种新的共聚焦显微镜，该显微镜将扩大设施，并允许所有研究人员使用新技术。 V1.6将维护并将用作教学工具，适用于有传统需求和溢出需求的用户。由于我们目前高于容量，升级将不会使更多成员受益，并允许大学提供更大的用户群。我们要求使用LSM＃18 405/ar/dual hene/3 pmt和4个内部检测器通道的axiovert上安装的v3.2 LSM 510。我们还要求一个机动阶段，以支持当前正在进行的大量实时研究和固定研究。生理，多时间系列和3D分析软件对于支持研究者的发展以及包括带有多个触发点的Ca2+成像时也至关重要。该列表还包括与我联系但无法使用当前工具的调查人员，因为它不支持他们的实验需求。已经收集了有关现有显微镜的广泛数据，现在用户受到技术的局限性以及它用于容量的局限性的限制。主要用户通常会随着时间的推移进行活细胞成像实验，以评估开发或遵循对伤害的反应。医学院的院长支持该工具作为大学的设施，并按照该工具支持全职人员每天进行共享。迪恩·麦卡汉（Dean McCahan）还支持支持共识系统和工作站所需的服务合同。