Zeiss LSM 510 META Confocal Microscope

蔡司 LSM 510 META 共焦显微镜

• 批准号: 6877461
• 负责人: STANLEY C FROEHNER
• 金额: $ 46.19万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877461
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; confocal scanning microscopy; electrophysiology; fluorescence resonance energy transfer; protein structure function

DESCRIPTION (provided by applicant): This proposal requests support for a confocal microscope with state of the art features for multi-fluorescent protein studies, FRET imaging and electrophysiological studies. The Zeiss LCS 510 META confocal microscope will provide technology that is currently unavailable to and is pivotal for the research effort of eleven independent laboratories in the biomedical sciences. Research programs in all eleven laboratories are now at a critical phase where long term, reliable, and frequent access to this equipment is essential for further progress. All investigators are NIH-funded with research programs that include the following areas of investigation: mechanisms of ion channel and transporter function, synaptogenesis, structure and function of opioid receptors, signal transduction pathways in early embryonic development, calcium signaling in cardiac and vascular smooth muscle, the regulation of expression and localization of muscarinic acetylcholine receptors, stem cell repair of cardiac infarcts, bacterial pathogenesis and metabolism and cellular actions of cannabinoids in neurons. Although the research in these laboratories is primarily basic science in nature, it also has major and direct implications for numerous human diseases and maladies, including cardiac and vascular disease, cancer, muscular dystrophy, bacterial pathogenesis, defects of the visual system and drug addiction and abuse. The core investigators represent five separate departments in two schools. The broad range of projects proposed by the primary user group is representative of a larger group of potential users of the new microscope system. The requested instrument would be placed in the established Keck Imaging Facility located on the University of Washington campus and would be made available to other investigators when it is not being used by the core group of researchers. The operation and management of this equipment, the internal advisory committee and institutional support for the confocal microscope are described. The Keck Imaging Center and its director, Greg Martin, are widely known and valued at the University of Washington and its affiliated institutions, thus assuring that the new instrument and its state-of-the- art capabilities will have a broadly based clientele.

描述（由申请人提供）：该提案要求支持共聚焦显微镜，并具有最先进的特征，用于多荧光蛋白研究，FRET成像和电生理研究。 Zeiss LCS 510 Meta共聚焦显微镜将提供目前无法使用的技术，并且对于生物医学科学中11个独立实验室的研究工作至关重要。现在，所有11个实验室的研究计划都处于关键阶段，长期，可靠和频繁访问该设备对于进一步的进步至关重要。 All investigators are NIH-funded with research programs that include the following areas of investigation: mechanisms of ion channel and transporter function, synaptogenesis, structure and function of opioid receptors, signal transduction pathways in early embryonic development, calcium signaling in cardiac and vascular smooth muscle, the regulation of expression and localization of muscarinic acetylcholine receptors, stem cell repair of cardiac infarcts, bacterial pathogenesis and大麻素在神经元中的代谢和细胞作用。尽管这些实验室的研究本质上主要是基础科学，但它对许多人类疾病和疾病也具有重大和直接的影响，包括心脏和血管疾病，癌症，肌肉营养不良，细菌发病机理，视觉系统和药物成瘾和滥用的缺陷。核心调查人员在两所学校中代表五个单独的部门。主要用户组提出的广泛项目代表了新显微镜系统的较大潜在用户。所请求的仪器将放置在位于华盛顿大学校园的既定凯克成像设施中，并在核心研究人员没有使用时将其提供给其他研究人员。描述了该设备的操作和管理，内部咨询委员会和共聚焦显微镜的机构支持。凯克成像中心及其董事格雷格·马丁（Greg Martin）在华盛顿大学及其附属机构中广为人知，并珍视，从而确保新工具及其最先进的能力将具有广泛的客户。