JOEL MODEL JEM-1230 TRANSMISSION ELECTRON MICROSCOPE

JOEL JEM-1230 型透射电子显微镜

• 批准号: 6874092
• 负责人: IAN G MACARA
• 金额: $ 41.18万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6874092
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; cell biology; microbiology; transmission electron microscopy

DESCRIPTION (provided by applicant): Funds are requested to support purchase of a new JEOL transmission electron microscope, model JEM- 1230, with an AMT-X100 high-resolution digital camera system. This microscope will be administered and maintained by the Advanced Microscopy Facility (AMF) within the School of Medicine (SOM). An advisory committee, consisting of 8 senior faculty plus the Technical Director of the AMF, will oversee the instrument. The principal investigator is the AMF Faculty Director and chair of the advisory committee. The AMF is a core fee-for service facility for users throughout the Medical School. About 72 principal investigators and their research groups use these facilities each year. Currently the AMF operates two electron microscopes that each are more than 20 years old, and are no longer reliable. There are several other electron microscopes within the SOM and elsewhere on campus, but they are in individual laboratories and are not generally available to NIH-funded investigators. The JEM-1230 TEM will fill a critical need within the University of Virginia for reliable, high resolution, high contrast transmission electron microscopy. It will be particularly valuable in the multi-user setting of the AMF because users can each store personalized settings. The improved imaging features will be important for several projects that use rotary shadowing to determine the overall conformation of protein complexes, and for fragile specimens that are sensitive to beam damage. The microscope will be housed in newly renovated space dedicated to the AMF. The SOM also supports the salaries of the Technical Director and a laboratory specialist in the AMF. There will be 5 NIH-funded primary users, and at least 4 minor users. Instrument time will be reserved by users on a Web-based calendar. The hourly fee and scheduling will be determined by the advisory committee and PI.

描述（由申请人提供）： 请求资金支持购买新型 JEOL 透射电子显微镜，型号为 JEM-1230，配有 AMT-X100 高分辨率数码相机系统。该显微镜将由医学院 (SOM) 内的高级显微镜设施 (AMF) 管理和维护。由 8 名高级教师和 AMF 技术总监组成的咨询委员会将监督该仪器。主要研究者是 AMF 教员主任兼咨询委员会主席。 AMF 是面向整个医学院用户的核心收费服务设施。每年约有 72 名主要研究人员及其研究小组使用这些设施。目前，AMF 拥有两台电子显微镜，每台都有 20 多年的历史，不再可靠。 SOM 内和校园其他地方还有其他几台电子显微镜，但它们位于单独的实验室中，一般不提供给 NIH 资助的研究人员使用。 JEM-1230 TEM 将满足弗吉尼亚大学对可靠、高分辨率、高对比度透射电子显微镜的关键需求。这在 AMF 的多用户设置中尤其有价值，因为每个用户都可以存储个性化设置。改进的成像功能对于使用旋转阴影确定蛋白质复合物整体构象的几个项目以及对光束损伤敏感的易碎样本来说非常重要。该显微镜将安置在新装修的 AMF 专用空间中。 SOM 还支持 AMF 技术总监和实验室专家的薪资。将有 5 名 NIH 资助的主要用户，以及至少 4 名次要用户。用户将在基于网络的日历上保留仪器时间。每小时的费用和时间安排将由咨询委员会和 PI 确定。