Purchase of an imaging flow cytometer 2016

2016年购买成像流式细胞仪

• 批准号: 9273704
• 负责人: Rick L Tarleton
• 金额: $ 45.38万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-04-15 至 2018-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9273704
• 关键词: Adipose tissue; Affect; African Trypanosomiasis; Agriculture; Biotechnology; Cattle; Cell Cycle; Cell Cycle Progression; Cell Nucleus; Cell physiology; Cells; Chagas Disease; Communicable Diseases; Communities; Cytometry; Detection; Disease; Drug resistance; Environmental Impact; Environmental Pollutants; Flow Cytometry; Fluorescence; Funding; Genes; Image; Immunology; Infection; Infectious Diseases Research; Institution; Investigation; Knowledge; Laboratories; Latin America; Malaria; Microscopy; Obesity; Pluripotent Stem Cells; Request for Proposals; Research; Resource Sharing; Speed; Stem cells; Universities; Viral; Virus Replication; cancer therapy; drug development; high resolution imaging; insight; instrument; nagana; programs; stem cell differentiation

Project Summary/Abstract: This proposal requests funds to purchase an ImageStream Mark II imaging flow cytometer for the Cytometry Shared Resource Laboratory (CSRL) at the University of Georgia. The ImageStream combines the speed, sensitivity, and fluorescence detection capabilities of flow cytometry with the detailed imaging and functional insights of microscopy. This instrument produces multiple, high- resolution images of every cell directly in flow, including brightfield and darkfield and up to 10 fluorescent markers. The CTEGD Cytometry SRL is the central flow cytometry facility for UGA and is housed within and staffed by the UGA Center for Tropical and Emerging Global Diseases (CTEGD). For more than 15 years the CTEGD CSRL has served the entire university community and surrounding biotechnology companies as well as regional academic institutions. Major users of the facility include laboratories with research foci in infectious diseases, stem cell differentiation, environmental impacts on cellular functions and obesity. Among infectious disease research programs to be supported by this purchase are investigations of Chagas disease, a disease that affects >20 million people in Latin America and the U.S., African sleeping sickness and Nagana, an infection of cattle that results in ~$4 billion in agricultural loss per year, and malaria, one of the “big 3” infectious disease killers. Viral immunology is the focus of 2 of our major users who are studying the interaction between host genes and viral replication as it relates to drug resistance. The ImageStream will allow stem cell and cellular biologists to better understand the cell cycle progression of pluripotent stem cells and the impact of new cancer therapies, as well as to correlate the cell cycle with the effects of environmental pollutants. The knowledge obtained by studying adipose nuclei is projected to facilitate drug development in the field of obesity. Thus, we expect the ImageStream to have a broad impact on the diverse research across the UGA campus.

项目摘要/摘要： 该提案要求资金购买 ImageStream Mark II 成像流程 细胞计数共享资源实验室 (CSRL) 的细胞计数仪 佐治亚大学的 ImageStream 结合了速度、灵敏度和 流式细胞仪的荧光检测能力和详细成像 该仪器产生多种、高通量的和功能性的见解。 直接流动中每个细胞的分辨率图像，包括明场和暗场 CTEGD Cytometry SRL 是中心流程，最多可有 10 个荧光标记。 佐治亚大学细胞计数设施，位于佐治亚大学中心内并由其配备人员 热带和新发全球疾病 (CTEGD) 超过 15 年。 CTEGD CSRL 为整个大学社区及周边地区提供服务 生物技术公司以及地区学术机构的主要用户。 该设施包括专注于传染病、干细胞研究的实验室 细胞分化、环境对细胞功能的影响和肥胖。 此次购买将支持的传染病研究项目 正在调查恰加斯病，这种疾病影响了超过 2000 万人 拉丁美洲和美国、非洲昏睡病和纳加纳感染 每年造成约 40 亿美元的农业损失，而疟疾是造成农业损失的原因之一 “三大”传染病杀手 病毒免疫学是我们其中两个的重点。 研究宿主基因和病毒之间相互作用的主要用户 复制与耐药性有关，ImageStream 将允许干细胞进行复制。 和细胞生物学家更好地了解细胞周期进程 多能干细胞和新癌症疗法的影响，以及 将细胞周期与环境污染物的影响联系起来。 通过研究脂肪核获得的知识预计将有助于药物开发 因此，我们期望 ImageStream 能够在肥胖领域有所发展。 对整个佐治亚大学校园的多元化研究产生了广泛影响。