Purchase a Beckman Coulter MoFlo Astrios Flow Cytometer

购买 Beckman Coulter MoFlo Astrios 流式细胞仪

基本信息

批准号：
8639790
负责人：
Luis J Montaner
金额：
$ 59.49万
依托单位：
WISTAR INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-07-01 至 2015-06-30
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): This is a proposal to acquire a MoFlo Astrios flow cytometer/high-speed cell sorter to replace an old MoFlo Legacy (2001), increasing capacity and adding to capabilities of the current cell sorting facility, a key component for 10 major NIH-funded research programs. The rationale for this request rests on a careful assessment based on the following factors: 1. A need to improve cell sorting service capacity in the Flow Cytometry Facility as crucial for the current NIH-funded investigators and the anticipated growth in Wistar Institute programs. 2. One of 2 sorters, the FACSAria II, is capable of 16 fluorochrome/4 laser detection while the existing MoFlo allows for only five fluorochromes. Upgrading would allow greater flexibility and efficiency for investigators moving from multi-color analysis to sorting, not restricting them to only one sorter. 3. Experiments requiring bio-safety containment at levels greater than BSL-1 are currently restricted to the FACSAria II, resulting in scheduling bottlenecks. The proposed new cell sorter would be appropriately equipped to allow for BSL-2+ sorting. 4. Fluorescent protein sorting is currently limited, given the lack of appropriate laser excitation. The proposed sorter would have a laser in the green/yellow range for optimal excitation of fluorescent proteins such as mCherry, mTomato, etc. 5. The proposed sorter would be capable of six-way sorting, providing the ability to separate more populations than the current four, along with the ability to sort into slides or plates up to 1536 wells. 6. Te existing MoFlo Legacy is still capable of performing well and used on a mostly daily basis, and is preferred by many for its speed and sample recovery, but is suffering from more frequent and increasing amounts of downtime due to age-related repairs. This results in increasing amounts of operator time and places NIH-funded projects at risk due to the potential for longer downtimes due to availability of only one sorter. While major overhaul and enhancement is an option, the expense involved would be better applied into a new instrument. This well-regarded facility, established over 30 years ago, is currently staffed by three experienced technicians and monitored by a faculty user committee. Many investigators at Wistar and neighboring institutions routinely use the cell sorting services, with the existing sorters often heavily booked with occasional backlogs due to capacity limitations as discussed above. Therefore, we propose to upgrade sorting capacity to maintain state- of-the-art support for 10 major and 8 minor users, with 41 R01/P01/U01 NIH-funded research awards, through purchase of a well-equipped MoFlo Astrios high-speed sorter, with appropriate bio-safety level containment, six-way sorting, and the ability to sort single cells directly into tissue culture plates or slides.

描述（由申请人提供）：这是一项购买 MoFlo Astrios 流式细胞仪/高速细胞分选仪的提案，以取代旧的 MoFlo Legacy (2001)，增加容量并增强当前细胞分选设施（关键组件）的能力NIH 资助的 10 个主要研究项目。这一请求的理由基于基于以下因素的仔细评估： 1. 需要提高流式细胞术设施的细胞分选服务能力，这对于当前 NIH 资助的研究人员和 Wistar 研究所项目的预期增长至关重要。 2. 2 种分选机之一 FACSAria II 能够检测 16 种荧光染料/4 种激光，而现有的 MoFlo 只能检测 5 种荧光染料。升级将为研究人员从多色分析转向分选提供更大的灵活性和效率，而不是限制他们只能使用一台分选机。 3. 需要高于BSL-1级别的生物安全遏制的实验目前仅限于FACSAria II，导致调度瓶颈。拟议的新细胞分选仪将适当配备以允许 BSL-2+ 分选。 4. 由于缺乏适当的激光激发，荧光蛋白分选目前受到限制。所提议的分选机将具有绿/黄范围内的激光器，以最佳激发荧光蛋白（例如 mCherry、mTomato 等）。 5. 所提议的分选机将能够进行六向分选，能够比现有的分选机分离更多的群体。目前为 4 个，并且能够对多达 1536 个孔的载玻片或板进行分类。 6. 现有的 MoFlo Legacy 仍然能够良好地运行并在大多数情况下日常使用，并且因其速度和样本恢复而受到许多人的青睐，但由于与老化相关的维修而导致停机时间变得更加频繁且越来越多。这会导致操作员时间增加，并使 NIH 资助的项目面临风险，因为由于只有一台分拣机，可能会导致更长的停机时间。虽然大修和增强是一种选择，但所涉及的费用最好应用于新仪器。这个备受推崇的设施成立于 30 多年前，目前由三名经验丰富的技术人员组成，并由教师用户委员会进行监督。 Wistar 和邻近机构的许多研究人员经常使用细胞分选服务，由于如上所述的容量限制，现有的分选机经常被大量预订，偶尔会出现积压。因此，我们建议升级分选能力，通过购买装备精良的 MoFlo Astrios 高性能设备，为 10 个主要用户和 8 个次要用户提供最先进的支持，并获得 41 个 R01/P01/U01 NIH 资助的研究奖项。快速分选机，具有适当的生物安全级别密封、六向分选，并且能够将单细胞直接分选到组织培养板或载玻片中。