Hybrid 3D bioprinting systems for fabricating heterogeneous, vascularized tissue constructs

用于制造异质血管化组织结构的混合 3D 生物打印系统

• 批准号: RGPIN-2020-04559
• 负责人: Kim, Keekyoung
• 金额: $ 2.84万
• 依托单位: University of Calgary
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=759763
• 关键词: Hybrid; 3D; bioprinting; systems; fabricating

While traditional 2D printers print on a flat surface, 3D printers enable the addition of another dimension in the vertical direction to build a complete 3D object in a layer-by-layer fashion. Analogous to this process, bioprinting seeks to deposit layers of biomaterials to build 3D body parts. Harvesting stem cells from a transplant recipient and printing them to build a replacement organ could help solve current organ transplantation problems such as long wait times and the immune rejection. However, existing 3D bioprinting technology is limited when it comes to printing heterogeneous tissues and organs with blood vessels, which are essential for the supply of oxygen and nutrients to cells that are constituent of these structures. This research project aims to develop a 3D bioprinting system that is capable of printing physically heterogeneous tissue structures with blood vessel networks, that are more reminiscent of natural tissues. The demand for fabricating such tissues has set the basis for the long-term research goal, which is to develop and optimize advanced 3D bioprinting systems to replicate the complex structures of natural tissues and to eventually realize the printing of whole organs. To accomplish this long-term goal, specific short-term objectives of this program include: (i) The investigation of a novel grayscale stereolithographic 3D bioprinting method to fabricate physically heterogeneous tissue scaffolds; (ii) The investigation of a laser diode-based extrusion bioprinting method to print hollow fibrous scaffolds for blood vessel regeneration; and (iii) The development of a method to fabricate heterogeneous, vascularized tissue constructs by integrating the stereolithographic and extrusion 3D bioprinting functionalities. This research program will make important contributions to the field of advanced biomanufacturing by enabling the printing of increasingly realistic complex tissue structures. Utilizing the unique advantages of the novel hybrid 3D bioprinting system, the proposed research will result in the creation of innovative 3D bioprinting technologies, new methods and design guidelines for tissue scaffolds, and first-of-their-kinds 3D bioprinting systems suitable for practical application. These intelligent, hybrid bioprinting platforms will find immediate application in the fields of regenerative medicine and drug discovery, thus benefiting both the health and economy of Canadian society. The research program will create new technological options in providing therapeutic treatments to Canadians suffering from various diseases and will also spark the creation of new jobs through start-up companies and technology transfer to Canadian companies. Moreover, this program will also provide interdisciplinary training to HQPs and make them equip them with required academic and professional skills for a long-term impact and new initiatives in advanced biomanufacturing and healthcare technologies.

尽管传统的2D打印机在平坦的表面上打印，但3D打印机可以在垂直方向上添加另一个维度，以逐层方式构建完整的3D对象。类似于此过程，生物打印试图沉积生物材料层以建造3D身体部位。从移植受者中收集干细胞并将其打印以构建替代器官，这可以帮助解决当前器官移植问题，例如长时间等待时间和免疫排斥。但是，现有的3D生物打印技术在印刷异质组织和具有血管的器官时受到限制，这对于这些结构构成的细胞的氧气和养分至关重要。该研究项目旨在开发一个3D生物打印系统，该系统能够用血管网络打印物理异质组织结构，这更让人联想到天然组织。制造此类组织的需求为长期研究目标树立了基础，即开发和优化先进的3D生物打印系统，以复制自然组织的复杂结构，并最终实现整个器官的印刷。为了实现这一长期目标，该计划的特定短期目标包括：（i）研究一种新型的灰度立体镜3D生物打印方法，以制造物理上异质的组织支架； （ii）研究基于激光二极管的挤出生物打印方法，以打印空心纤维支架以进行血管再生； （iii）开发一种通过整合立体造影和挤出3D生物打印功能来制造异质的，血管化组织构建体的方法。 该研究计划将通过印刷日益现实的复杂组织结构来为高级生物制造领域做出重要贡献。利用新型混合3D生物打印系统的独特优势，拟议的研究将导致创建创新的3D生物打印技术，用于组织支架的新方法和设计指南，以及首次实现的3D生物包装系统。这些智能的混合生物打印平台将在再生医学和药物发现领域立即应用，从而使加拿大社会的健康和经济受益。该研究计划将创建新的技术选择，以为患有各种疾病的加拿大人提供治疗治疗，还将通过创业公司和技术转移到加拿大公司创造新的就业机会。此外，该计划还将为HQP提供跨学科的培训，并使他们为他们提供长期影响和高级生物制造和医疗保健技术的新计划所需的学术和专业技能。