Novel minimally-invasive in-situ 3D bioprinting platform for cardiac regeneration

用于心脏再生的新型微创原位3D生物打印平台

• 批准号: EP/X027287/2
• 负责人: Molly Stevens
• 金额: --
• 依托单位: University of Oxford
• 依托单位国家: 英国
• 项目类别: Fellowship
• 财政年份: 2024
• 资助国家: 英国
• 起止时间: 2024 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FX027287%2F2
• 关键词: Novel; minimally; invasive; situ; 3D; Novel; minimally; invasive; situ; 3D

Currently, cardiac disorders (e.g., infarction, arrythmia, ischemia, etc.) are one of the leading causes of death according to WHO. In this project I aim to use interdisciplinary approaches to develop a novel in-situ 3D bioprinting (inSituBioprint) platform for cardiac regeneration, to directly fabricate patient-specific cardiac patches on host tissue. This will produce a new generation of 3D printing method for automatic implantation, which will drastically improve the surgical outcomes and patients' well-being. In the proposed study, a minimally-invasive 3D bioprinting platform will be designed to access tissue only through small incision, which fully recapitulates the concept of laparoscopy (keyhole surgery). Rather than following pre-planned printing paths, the proposed printer will sense, learn, and adapt to the curved surface during printing, and the height of the nozzle-substrate gap will be determined via impedance spectroscopic sensing and machine learning (ML) facilitated spectra analysis. Based on this platform, the conductive copolymer will be synthesized and optimised for in situ printing. Finally, I will investigate the therapeutic efficacy and regenerative potential of the conductive cardiac patch using three cardiac models: nonviable porcine heart (dimensionally similar to human heart), 3D cardiomyocyte culture, and cryoinjured arrythmia myocardial slice model. The proposed study will validate a clinically-relevant 3D bioprinting technique to accelerate the translation of surgical robotics and tissue regeneration, and eventually to benefit patient undergoing surgeries.

目前，根据世卫组织（WHO），心脏疾病（例如，梗塞，雅利氏症，缺血等）是死亡的主要原因之一。在这个项目中，我旨在使用跨学科的方法来开发一种新型的基于心脏再生的现场3D生物打印（Insitubioprint）平台，以直接在宿主组织上直接制造患者特定的心脏斑块。这将产生新一代的3D打印方法，用于自动植入，这将大大改善手术结果和患者的福祉。在拟议的研究中，最小侵入性的3D生物打印平台将仅通过小切口访问组织，该切口完全概括了腹腔镜概念（钥匙孔手术）。拟议的打印机不会遵循预先计划的打印路径，而是会在打印过程中感知，学习和适应弯曲的表面，并且将通过阻抗光谱传感和机器学习（ML）来确定喷嘴覆盖物间隙的高度促进分析。基于此平台，将合成和优化用于原位打印的导电共聚物。最后，我将使用三种心脏模型研究导电性心脏斑块的治疗功效和再生潜力：不可生存的猪心（与人心脏的尺寸相似），3D心肌细胞培养以及腐烂的亚利氏菌心肌切片模型。拟议的研究将验证一种与临床上的3D生物打印技术，以加速手术机器人和组织再生的翻译，并最终使接受手术的患者受益。