Novel minimally-invasive in-situ 3D bioprinting platform for cardiac regeneration

用于心脏再生的新型微创原位3D生物打印平台

• 批准号: EP/X027287/2
• 负责人: Molly Stevens
• 金额: --
• 依托单位: University of Oxford
• 依托单位国家: 英国
• 项目类别: Fellowship
• 财政年份: 2024
• 资助国家: 英国
• 起止时间: 2024 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FX027287%2F2
• 关键词: Novel; minimally; invasive; situ; 3D

Currently, cardiac disorders (e.g., infarction, arrythmia, ischemia, etc.) are one of the leading causes of death according to WHO. In this project I aim to use interdisciplinary approaches to develop a novel in-situ 3D bioprinting (inSituBioprint) platform for cardiac regeneration, to directly fabricate patient-specific cardiac patches on host tissue. This will produce a new generation of 3D printing method for automatic implantation, which will drastically improve the surgical outcomes and patients' well-being. In the proposed study, a minimally-invasive 3D bioprinting platform will be designed to access tissue only through small incision, which fully recapitulates the concept of laparoscopy (keyhole surgery). Rather than following pre-planned printing paths, the proposed printer will sense, learn, and adapt to the curved surface during printing, and the height of the nozzle-substrate gap will be determined via impedance spectroscopic sensing and machine learning (ML) facilitated spectra analysis. Based on this platform, the conductive copolymer will be synthesized and optimised for in situ printing. Finally, I will investigate the therapeutic efficacy and regenerative potential of the conductive cardiac patch using three cardiac models: nonviable porcine heart (dimensionally similar to human heart), 3D cardiomyocyte culture, and cryoinjured arrythmia myocardial slice model. The proposed study will validate a clinically-relevant 3D bioprinting technique to accelerate the translation of surgical robotics and tissue regeneration, and eventually to benefit patient undergoing surgeries.

据世界卫生组织称，目前，心脏疾病（例如梗塞、心律失常、缺血等）是导致死亡的主要原因之一。在这个项目中，我的目标是利用跨学科方法开发一种用于心脏再生的新型原位 3D 生物打印 (inSituBioprint) 平台，以直接在宿主组织上制造患者特异性心脏贴片。这将产生新一代的自动植入3D打印方法，将大大改善手术结果和患者的福祉。在拟议的研究中，微创3D生物打印平台将被设计为仅通过小切口进入组织，这完全概括了腹腔镜手术（锁孔手术）的概念。所提出的打印机将在打印过程中感知、学习和适应弯曲表面，而不是遵循预先计划的打印路径，并且喷嘴与基材间隙的高度将通过阻抗光谱传感和机器学习（ML）促进的光谱来确定分析。基于该平台，将合成并优化用于原位打印的导电共聚物。最后，我将使用三种心脏模型研究导电心脏贴片的治疗功效和再生潜力：不可存活的猪心脏（尺寸与人类心脏相似）、3D 心肌细胞培养物和冷冻损伤心律失常心肌切片模型。拟议的研究将验证临床相关的 3D 生物打印技术，以加速手术机器人和组织再生的转化，并最终造福接受手术的患者。