PERSONALIZED COVID-19 DIAGNOSTICS WITH IMMUNOPLASMONIC MICROSCOPY

利用免疫等离子显微镜进行个性化 COVID-19 诊断

• 批准号: 555170-2020
• 负责人: Meunier, Michel
• 金额: $ 3.64万
• 依托单位: École Polytechnique de Montréal
• 依托单位国家: 加拿大
• 项目类别: Alliance Grants
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=705779
• 关键词: PERSONALIZED; COVID; 19; DIAGNOSTICS; IMMUNOPLASMONIC

At this time, healthcare system is facing heavy challenges requiring new and urgent tools to handle and relief severe impacts of COVID-19 outbreak. Personalized access to affordable, high-quality diagnostic tools for the detection of infectious diseases, including SARS-CoV-2, in resource-limited settings and outside the hospital environment, is imperative. In this project, we propose an alternative and promising solution for rapid clinical and personalized COVID-19 diagnosis. High efficiency and sensitivity of the proposed device is ensured by the use of an elaborated Imaging Localized Surface Plasmon Resonance (ILSPR) biosensing technology. The key innovation here is the dual utilization of specifically functionalized proprietary own immunoplasmonic nanoparticles (NPs). Due to their unique optical and chemical properties, plasmonic NPs, which are precisely functionalized by specific biomolecules can perform as (I) selective biomarkers of target viruses (i.e., the detection of expressed spike proteins), and as (II) optically active tags easily detectable and quantifiable under our simplified optical imaging system (i.e., dark-field side-illumination microscopy developed in our laboratory). Our well-organized research team, including, chemists, biologists, engineers, and clinical biochemist from the Maisonneuve-Rosemont Hospital with a collaboration of the innovation company (Allied Scientific Medical) joint their efforts to develop a professional cost-effective, plasmonic biosensing system to diagnose patients outside of centralized facilities. Taking advantage of the widespread distribution of personal electronic devices (i.e., smartphones and tablets) equipped with an effective optical sensor and communication capability, the proposed ILSPR plasmonic biosensor can also be considered as the hand-held portable personalized diagnostic device capable to obtain accurate results in less than 10 minutes and dispatches it to specialists for a precise analysis or confirmation through a telemedicine network.

目前，医疗保健系统正面临着艰巨的挑战，需要新的和紧急的工具来处理和救济Covid-19-19-19。必须使用个性化访问可负担的高质量诊断工具，用于检测传染病，包括在资源有限的环境中和医院环境外，包括SARS-COV-2在内。在这个项目中，我们为快速临床和个性化的Covid-19诊断提出了一种替代和有希望的解决方案。通过使用详细成像的局部表面等离子体共振（ILSPR）生物传感技术来确保所提出的设备的高效率和灵敏度。这里的关键创新是对特定功能化专有自身免疫质纳米颗粒（NP）的双重利用。 Due to their unique optical and chemical properties, plasmonic NPs, which are precisely functionalized by specific biomolecules can perform as (I) selective biomarkers of target viruses (i.e., the detection of expressed spike proteins), and as (II) optically active tags easily detectable and quantifiable under our simplified optical imaging system (i.e., dark-field side-illumination microscopy developed in our laboratory).我们组织良好的研究团队，包括Maisonneuve-Rosemont医院的化学家，生物学家，工程师，工程师和临床生物化学家与创新公司（Allied Scientific Medical）合作的合作，共同开发专业的成本有效的等离子，等离子化等离子，等离子化等离子，等离子化等离子，等离子等离子，以诊断集中设施以外的患者。利用配备有效的光学传感器和通信能力的个人电子设备（即智能手机和平板电脑）的广泛分布，提议的ILSPR等离子体生物传感器也可以被视为手持式便携式性个性化诊断设备，能够在少于10分钟内获得准确的诊断设备，并通过少于10分钟的网络来派遣专家，并通过精确的分析进行派遣。