Improving the reliability of cytology diagnosis with immunoplasmonic multiplex labeling

利用免疫等离子多重标记提高细胞学诊断的可靠性

• 批准号: 523671-2018
• 负责人: Meunier, Michel
• 金额: $ 14.64万
• 依托单位: École Polytechnique de Montréal
• 依托单位国家: 加拿大
• 项目类别: Collaborative Health Research Projects
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=694560
• 关键词: Improving; reliability; cytology; diagnosis; immunoplasmonic

Most cancer diagnoses are first established using a biopsy, and as such, biopsies are criticalto the health care of Canadians. Different types of biopsies are performed to achieve adiagnosis, but physicians do prefer fine-needle aspiration cytology, hereafter referred to ascytology, because it is associated with fewer side effects for the patients. Accordingly, thenumber of cytology performed annually is increasing for many organs such as lung andpancreas. Cytology is evaluated by pathologists, who are using different tools to refine theirdiagnoses. The objective of the most commonly used tool is to detect proteins in the cellswhich are specific for cancer. The multiple preparation steps that have to be performed tocolour those proteins can destroy said proteins and therefore affect the reliability of the tool.Our objective is therefore to bypass the preparation steps by using metallic nanoparticles toidentify the proteins without degrading them, thus improving the reliability of the diagnosis.Furthermore, we propose to use specific laser-based technologies to detect the metallicnanoparticles. This project may be considered pioneering as clinical validation on humancytology samples will ensure highly beneficial accuracy of diagnosis and optimal selection oftreatment, consequently improving the health of Canadian patients. To achieve this innovativeresearch project, we put together an excellent multidisciplinary and complementary team ofinternationally renowned scientists and industrial partners coming from the full spectrum ofknowledge involved in this project. This team has already acquired a strong expertise inmetallic nanoparticles use and detection for biological diagnostics and we are recognized byour peers as protein detection experts. Our excellence is demonstrated by multiple patentsand declarations of inventions. Our industrial partner, Photon etc, completes our skills incommercialization of metallic nanoparticles and their detection.

大多数癌症诊断首先通过活检确定，因此，活检对于加拿大人的医疗保健至关重要。进行不同类型的活检来实现诊断，但医生确实更喜欢细针抽吸细胞学检查（以下称为细胞学检查），因为它对患者的副作用较少。因此，每年对肺和胰腺等许多器官进行的细胞学检查的数量正在增加。细胞学由病理学家评估，他们使用不同的工具来完善他们的诊断。最常用工具的目的是检测细胞中癌症特异性的蛋白质。为这些蛋白质着色而必须执行的多个制备步骤会破坏所述蛋白质，从而影响工具的可靠性。因此，我们的目标是通过使用金属纳米粒子来识别蛋白质而不降解它们来绕过制备步骤，从而提高工具的可靠性此外，我们建议使用特定的基于激光的技术来检测金属纳米颗粒。该项目可被认为是开创性的，因为对人类细胞学样本的临床验证将确保诊断的高度准确性和最佳治疗选择，从而改善加拿大患者的健康。为了实现这一创新研究项目，我们组建了一支优秀的多学科互补团队，由国际知名科学家和工业合作伙伴组成，他们拥有该项目所涉及的全方位知识。该团队已经在生物诊断的金属纳米颗粒使用和检测方面拥有强大的专业知识，我们被同行认可为蛋白质检测专家。多项专利和发明声明证明了我们的卓越品质。我们的工业合作伙伴 Photon 等完善了我们在金属纳米颗粒及其检测方面的商业化技能。