Improving the reliability of cytology diagnosis with immunoplasmonic multiplex labeling

利用免疫等离子多重标记提高细胞学诊断的可靠性

• 批准号: 523671-2018
• 负责人: Meunier, Michel
• 金额: $ 14.64万
• 依托单位: École Polytechnique de Montréal
• 依托单位国家: 加拿大
• 项目类别: Collaborative Health Research Projects
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=694560
• 关键词: Improving; reliability; cytology; diagnosis; immunoplasmonic

Most cancer diagnoses are first established using a biopsy, and as such, biopsies are criticalto the health care of Canadians. Different types of biopsies are performed to achieve adiagnosis, but physicians do prefer fine-needle aspiration cytology, hereafter referred to ascytology, because it is associated with fewer side effects for the patients. Accordingly, thenumber of cytology performed annually is increasing for many organs such as lung andpancreas. Cytology is evaluated by pathologists, who are using different tools to refine theirdiagnoses. The objective of the most commonly used tool is to detect proteins in the cellswhich are specific for cancer. The multiple preparation steps that have to be performed tocolour those proteins can destroy said proteins and therefore affect the reliability of the tool.Our objective is therefore to bypass the preparation steps by using metallic nanoparticles toidentify the proteins without degrading them, thus improving the reliability of the diagnosis.Furthermore, we propose to use specific laser-based technologies to detect the metallicnanoparticles. This project may be considered pioneering as clinical validation on humancytology samples will ensure highly beneficial accuracy of diagnosis and optimal selection oftreatment, consequently improving the health of Canadian patients. To achieve this innovativeresearch project, we put together an excellent multidisciplinary and complementary team ofinternationally renowned scientists and industrial partners coming from the full spectrum ofknowledge involved in this project. This team has already acquired a strong expertise inmetallic nanoparticles use and detection for biological diagnostics and we are recognized byour peers as protein detection experts. Our excellence is demonstrated by multiple patentsand declarations of inventions. Our industrial partner, Photon etc, completes our skills incommercialization of metallic nanoparticles and their detection.

大多数癌症诊断首先是使用活检建立的，因此，活检对加拿大人的医疗保健是至关重要的。进行了不同类型的活检以实现申请诊断，但是医生确实更喜欢细针细胞学，以后称为狂热学，因为它与患者的副作用更少有关。因此，对于许多器官，例如肺和胰腺，每年进行的细胞学研究都在增加。细胞学由病理学家评估，他们正在使用不同的工具来完善其诊断。最常用的工具的目的是检测Cellswhich中的蛋白质是特定于癌症的。 The multiple preparation steps that have to be performed tocolour those proteins can destroy said proteins and therefore affect the reliability of the tool.Our objective is therefore to bypass the preparation steps by using metallic nanoparticles toidentify the proteins without degrading them, thus improving the reliability of the diagnosis.Furthermore, we propose to use specific laser-based technologies to detect the metallicnanoparticles.该项目可能被认为是开拓性的，因为对HumancyTology样本的临床验证将确保诊断和最佳选择的最佳选择，从而改善了加拿大患者的健康状况。为了实现这一Innovativeresearch项目，我们组建了一个出色的多学科和补充团队，由知名的科学家和工业合作伙伴来自该项目所涉及的全部知识。该团队已经获得了强大的专业知识，用于生物学诊断的非机构纳米颗粒使用和检测，我们被认为是byour同龄人是蛋白质检测专家。多个专利和发明声明证明了我们的卓越表现。我们的工业合作伙伴Photon等完成了我们对金属纳米颗粒的技能不商业化及其检测。