Improving the reliability of cytology diagnosis with immunoplasmonic multiplex labeling

利用免疫等离子多重标记提高细胞学诊断的可靠性

• 批准号: 523671-2018
• 负责人: Meunier, Michel
• 金额: $ 7.32万
• 依托单位: École Polytechnique de Montréal
• 依托单位国家: 加拿大
• 项目类别: Collaborative Health Research Projects
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=659694
• 关键词: Improving; reliability; cytology; diagnosis; immunoplasmonic

Most cancer diagnoses are first established using a biopsy, and as such, biopsies are critical**to the health care of Canadians. Different types of biopsies are performed to achieve a**diagnosis, but physicians do prefer fine-needle aspiration cytology, hereafter referred to as**cytology, because it is associated with fewer side effects for the patients. Accordingly, the**number of cytology performed annually is increasing for many organs such as lung and**pancreas. Cytology is evaluated by pathologists, who are using different tools to refine their**diagnoses. The objective of the most commonly used tool is to detect proteins in the cells**which are specific for cancer. The multiple preparation steps that have to be performed to**colour those proteins can destroy said proteins and therefore affect the reliability of the tool.**Our objective is therefore to bypass the preparation steps by using metallic nanoparticles to**identify the proteins without degrading them, thus improving the reliability of the diagnosis.**Furthermore, we propose to use specific laser-based technologies to detect the metallic**nanoparticles. This project may be considered pioneering as clinical validation on human**cytology samples will ensure highly beneficial accuracy of diagnosis and optimal selection of**treatment, consequently improving the health of Canadian patients. To achieve this innovative**research project, we put together an excellent multidisciplinary and complementary team of**internationally renowned scientists and industrial partners coming from the full spectrum of**knowledge involved in this project. This team has already acquired a strong expertise in**metallic nanoparticles use and detection for biological diagnostics and we are recognized by**our peers as protein detection experts. Our excellence is demonstrated by multiple patents**and declarations of inventions. Our industrial partner, Photon etc, completes our skills in**commercialization of metallic nanoparticles and their detection.

大多数癌症诊断首先通过活检确定，因此，活检对于加拿大人的医疗保健至关重要**。进行不同类型的活检来实现**诊断，但医生确实更喜欢细针抽吸细胞学检查，以下简称**细胞学检查，因为它对患者的副作用较少。因此，对于肺和**胰腺等许多器官来说，每年进行的细胞学检查数量正在增加。细胞学由病理学家评估，他们使用不同的工具来完善他们的**诊断。最常用工具的目的是检测细胞中的癌症特异性蛋白质**。为这些蛋白质着色而必须执行的多个制备步骤会破坏所述蛋白质，从而影响工具的可靠性。**因此，我们的目标是通过使用金属纳米颗粒来**识别蛋白质，从而绕过制备步骤，而无需降解它们，从而提高诊断的可靠性。**此外，我们建议使用特定的基于激光的技术来检测金属**纳米颗粒。该项目可被认为是开创性的，因为对人类细胞学样本的临床验证将确保诊断的高度准确性和治疗的最佳选择，从而改善加拿大患者的健康。为了实现这一创新**研究项目，我们组建了一支优秀的多学科互补团队，由**国际知名科学家和工业合作伙伴组成，他们拥有该项目所涉及的**知识。该团队已经在生物诊断的金属纳米粒子使用和检测方面获得了强大的专业知识，并且我们被同行认可为蛋白质检测专家。多项专利**和发明声明证明了我们的卓越品质。我们的工业合作伙伴 Photon 等完善了我们在金属纳米粒子及其检测商业化方面的技能。