Interaction of ultrasonically-stimulated microbubbles with biological membranes

超声波刺激微泡与生物膜的相互作用

• 批准号: RGPIN-2015-05941
• 负责人: Karshafian, Raffi
• 金额: $ 1.6万
• 依托单位: Ryerson University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2016
• 资助国家: 加拿大
• 起止时间: 2016-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=613839
• 关键词: Interaction; ultrasonically; stimulated; microbubbles; biological

Ideally, therapeutic drugs would be delivered entirely to the diseased tissue, sparring healthy tissues. Of particular interest to our group is the application of sound energy at megahertz frequencies in combination with microbubbles, tiny bubbles smaller than red blood cells, to selectively improve delivery of drugs to cancer cells. Microbubbles are micrometer size, gas-filled structures stabilized by a lipid or polymer shell. When exposed to therapeutic ultrasound, microbubbles can undergo large oscillations and disruption that can increase uptake of drug-molecules into biological cells, which were within the microbubble's vicinity. Our group will examine the interaction of ultrasound with microbubbles and its effect on live cells using a microscopy system that can acquire images of oscillating microbubbles at 2 million frames per second, fluorescent images of cells at one thousand frames per second while simultaneously listening to the sound emitted by microbubbles. Fundamental studies of the interaction of ultrasonically-stimulated microbubbles with biological membranes in real time will improve our understanding of underlying mechanisms and guide the optimization of ultrasound-potentiated cancer therapies.

理想情况下，治疗药物将完全输送到患病组织，而不影响健康组织。我们小组特别感兴趣的是应用兆赫频率的声能与微泡（比红细胞更小的微小气泡）相结合，以选择性地改善药物向癌细胞的输送。微泡是微米尺寸的、由脂质或聚合物壳稳定的充气结构。当暴露于治疗性超声波时，微泡会经历较大的振荡和破坏，从而增加微泡附近的生物细胞对药物分子的吸收。我们的小组将使用显微镜系统检查超声波与微泡的相互作用及其对活细胞的影响，该系统可以以每秒 200 万帧的速度获取振荡微泡的图像，以每秒 1000 帧的速度获取细胞的荧光图像，同时聆听声音由微气泡发射。对超声刺激微泡与生物膜相互作用的实时基础研究将提高我们对潜在机制的理解，并指导超声增强癌症疗法的优化。