Interaction dynamics and bioeffects of acoustically activated microbubbles with biological membranes

声激活微泡与生物膜的相互作用动力学和生物效应

基本信息

批准号：
386274-2010
负责人：
Karshafian, Raffi
金额：
$ 1.82万
依托单位：
Ryerson University
依托单位国家：
加拿大
项目类别：
Discovery Grants Program - Individual
财政年份：
2014
资助国家：
加拿大
起止时间：
2014-01-01 至 2015-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=550353
关键词：
Interaction dynamics bioeffects acoustically activated

项目摘要

Ideally, therapeutic drugs would be delivered entirely to diseased tissues, sparring healthy tissue. However, existing therapeutic drugs generally interact with both diseased and healthy tissues. Treatment effectiveness of cytotoxic drugs such as chemotherapy depends on the extent to which their constituents penetrate tissues and cells to reach their intended target and is limited by toxic side effects exerted on tissues and cells not associated with the disease. Of particular interest to our group is the ability of sound energy in combination with tiny bubbles, which are smaller than red blood cells, to non-invasively improve delivery of drugs to diseased cells and tissues deep with the body. Our group will examine the interaction of sound energy with bubbles and their effect on live cells with the aim of answering fundamental questions on the biophysical mechanisms involved, as this remains a subject of considerable investigation. Bubbles, exposed to sound energy at megahertz frequency (millions of oscillation per second) that undergo oscillation and/or violent collapse, will be characterized using acoustic methods and a high-speed camera (500,000 frames per second). Cells will be analyzed using a variety of biological methods. Improving our understanding of the processes underpinning the ability of ultrasound and bubbles to selectively and locally deliver therapeutic agents will allow optimization of drug-treatments and also lead to a better understanding of the strengths and limitations of this technology - ultrasound targeted delivery of drugs - with regards to its clinical use.

理想情况下，治疗药物将完全递送到患病的组织中，并散布健康的组织。但是，现有的治疗药物通常与患病和健康组织相互作用。细胞毒性药物（例如化学疗法）的治疗有效性取决于其成分穿透组织和细胞达到其预期靶标的程度，并且受到与疾病无关的组织和细胞的毒性副作用的限制。我们组特别感兴趣的是声音能量与小气泡相结合的能力（比红细胞都小的气泡）非侵入性地改善药物向患病细胞和组织的递送与身体深处的递送。我们的小组将检查声能与气泡的相互作用及其对活细胞的影响，目的是回答有关涉及的生物物理机制的基本问题，因为这仍然是大量研究的主题。通过声学方法和高速摄像头（每秒500,000帧）来表征气泡，暴露于Megahertz频率（每秒数百万振荡）的声音能量（每秒数百万振荡）。将使用多种生物学方法分析细胞。提高我们对超声和气泡有选择和本地提供治疗剂能力的过程的理解将允许对药物处理进行优化，并可以更好地理解该技术的优势和局限关于其临床用途。