Purchase of a small animal irradiator

购买小动物照射器

• 批准号: 472383-2015
• 负责人: Heinonen, Krista
• 金额: $ 10.93万
• 依托单位: Institut national de la recherche scientifique
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=564736
• 关键词: Purchase; small; animal; irradiator

Biological irradiators, using either X-ray or radioactive sources, are often used in cell biology to introduce DNA damage and to stop cell proliferation. In addition to the use of irradiation as a direct method to study the impact of DNA damage to cell growth and/or the mechanisms associated with DNA repair, irradiation has other, widespread, potential applications: 1) total body irradiation will wipe out the animal's immune system, which can then be replaced by cells of different genetic composition to study the mechanisms of blood cell development or of the immune response; 2) targeted irradiation can be used to sterilize mice to study infertility and the generation and production of sperm; 3) irradiation of bacterial pathogens can serve to determine roles of specific components, growth conditions, or regulatory pathways in stress resistance that could contribute to survival on foods and virulence; and 4) irradiation of cells in culture can be used to reduce the number of confounding variables from experimental systems including more than one cell type. In brief, access to irradiation sources is essential for a wide variety of research programs in natural sciences.

使用X射线或放射性源的生物辐照器通常用于细胞生物学中，以引入DNA损伤并阻止细胞增殖。除了使用辐照作为研究DNA损伤对细胞生长和/或与DNA修复相关的机制的影响的直接方法外，辐射还具有其他，广泛的潜在应用：1）总体辐照将消灭动物的动物免疫系统可以用不同遗传组成的细胞代替，以研究血细胞发育的机制或免疫反应的机制； 2）靶向辐射可用于对小鼠进行消毒以研究不孕症以及精子的产生和产生； 3）细菌病原体的辐照可以确定特定成分，生长条件或调节途径在抗压力性中的作用，这可能有助于食品和毒力生存； 4）细胞在培养物中的照射可用于减少来自包括多种细胞类型的实验系统的混杂变量的数量。简而言之，获得辐射来源对于自然科学的各种研究计划至关重要。