Non Human Primate Core

非人类灵长类核心

基本信息

批准号：
10619299
负责人：
Michael K Axthelm
金额：
$ 148.9万
依托单位：
OREGON HEALTH & SCIENCE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-09-22 至 2027-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10619299
关键词：
Animal Housing Animal Model Animals Autopsy CD8-Positive T-Lymphocytes Cells Clinical Clinical Data Clinical Management Communicable Diseases Complex Data Data Analyses Data Collection Elements Ensure Epitopes Event Goals HIV-1 Human Human Resources Immune Immunize Immunologics Infection Infrastructure Intercept Interleukin-15 Investigation Lead Leadership Macaca mulatta Mediating Mission Modeling Pathologic Performance Physiological Procedures Program Research Project Grants Protocols documentation Quality Control Records Research Research Personnel Risk Role SIV SIV Vaccines Safety Sampling Signal Transduction Specimen Handling Structure Supervision T-Lymphocyte Technical Expertise Tissues Vaccination Viral Virus Diseases Virus Replication Work efficacy evaluation efficacy research experience experimental study in vivo nonhuman primate novel programs response sample collection simian human immunodeficiency virus transcriptomics vector virtual

项目摘要

CORE B SUMMARY The Nonhuman Primate (NHP) Core seeks to consolidate supervision and performance of the NHP experimental protocols of the Program Project entitled “Immunologic and virologic basis of RhCMV/SIV vaccine-induced replication arrest efficacy” into a structured Core composed of highly experienced research personnel. The global objective of the Core is to provide leadership and technical expertise to ensure consistency and quality control in animal selection, execution of study protocols, application of experimental procedures, animal observations and data collection necessary to meet the objectives of Project and other Core lead investigators. To accomplish this, the Core will manage and directly supervise all NHP studies for the Project including: 1) animal selection; 2) animal housing and general husbandry; 3) experimental procedures and clinical management; 4) specimen collection and processing; 5) necropsy studies; and 6) acquisition and management of animal demographic, physiologic, clinical, and pathologic data. The overall objective of the Program Project is to determine the mechanisms responsible for complete arrest and subsequent clearance of nascent SIV infection in RhCMV/SIV- immunized rhesus macaques, including the requirement for MHC-E restricted epitope recognition for efficacy, how cells mediate replication arrest and the role of IL-15 signaling identified as a predictive transcriptomic signature. The NHP Core will provide the expertise and technical support required to insure successful completion of the multifaceted and extensive NHP research protocols supporting Project 1 – Immunologic and virologic characterization of RhCMV/SIV vaccine-mediated SIV “replication arrest” efficacy, Project 2 – Characterization of the in vivo T cell (and overall immune) interception of primary SIV infection after vaccination with differentially response programmed RhCMV/SIV vectors (MHC-E vs. MHC-II vs. MHC-Ia) and a conventional SIV vaccine, and Project 3 – Determination of the minimal MHC-E-restricted SIV epitope targeting required for RhCMV/SIV vaccine-mediated SIV “replication arrest” efficacy.

核心B总结非人类灵长类动物 (NHP) 核心旨在巩固 NHP 实验的监督和绩效题为“RhCMV/SIV 疫苗诱导的免疫学和病毒学基础”的项目方案复制抑制功效”纳入由全球经验丰富的研究人员组成的结构化核心。核心的目标是提供领导力和技术专业知识，以确保一致性和质量控制动物选择、研究方案的执行、实验程序的应用、动物观察实现项目和其他核心首席研究员的目标所必需的数据收集。为此，核心将管理和直接监督该项目的所有 NHP 研究，包括： 1) 动物选择； 2) 动物饲养和一般饲养； 3) 实验程序和临床管理； 4) 标本收集和处理；5) 尸检研究；以及 6) 动物人口统计数据的获取和管理，该计划项目的总体目标是确定生理、临床和病理数据。 RhCMV/SIV 中负责完全阻止和随后清除新生 SIV 感染的机制免疫恒河猴，包括需要 MHC-E 限制性表位识别才能发挥功效，细胞如何介导复制停滞以及 IL-15 信号传导作为预测转录组的作用 NHP 核心将提供确保成功所需的专业知识和技术支持。完成支持项目 1 – 免疫学和 RhCMV/SIV 疫苗介导的 SIV“复制停滞”功效的病毒学特征，项目 2 – 疫苗接种后体内 T 细胞（和整体免疫）拦截原发性 SIV 感染的表征具有差异反应编程的 RhCMV/SIV 载体（MHC-E 与 MHC-II 与 MHC-Ia）和传统 SIV 疫苗，以及项目 3 – 确定最小 MHC-E 限制性 SIV 表位靶向 RhCMV/SIV 疫苗介导的 SIV“复制停滞”功效所需。