Expanded SPF Rhesus Macaque Breeding Colony for AIDS Research

扩大 SPF 恒河猴繁殖群以进行艾滋病研究

• 批准号: 9765428
• 负责人: Michael K Axthelm
• 金额: $ 163.28万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-01 至 2020-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9765428
• 关键词: Acquired Immunodeficiency Syndrome; Adult; Animal Model; Animals; Breeding; Communicable Diseases; Development; Disease; Female; Funding; Genetic; Genotype; HIV Infections; Health Sciences; Homologous Gene; Human; Immune response; Immunity; Immunological Models; Immunology; Infectious Diseases Research; Macaca; Macaca mulatta; Major Histocompatibility Complex; Microbial Genetics; Modeling; Opportunistic Infections; Oregon; Pathogenesis; Primates; Production; Request for Applications; Research; Resistance; Resources; SIV; Target Populations; Universities; Vaccine Research; Viral; Viral Vaccines; Virus; Zoonoses; base; design; enzootic; germ free condition; improved; offspring; pre-clinical; simian human immunodeficiency virus; vaccine development; vector vaccine

OVERALL PROJECT SUMMARY “Expanded SPF Rhesus Macaque Breeding Colony for AIDS Research” The Indian rhesus macaque (Macaca mulatta) develops a disease that closely mimics human acquired immunodeficiency syndrome (AIDS) when infected by simian immunodeficiency virus (SIV) or chimeric simian- human immunodeficiency viruses (SHIV), and represents the best animal model for HIV infection. Preclinical vaccine development is heavily dependent on the SIV and SHIV rhesus macaque models. The value and utility of the model are markedly enhanced by improving the level of microbial and genetic characterization. Macaques free of ubiquitous viruses that are homologues of human viruses responsible for opportunistic infections are essential for a growing number of AIDS-related opportunistic infection models and for viral vaccine vector development based on these agents. The utility of macaque models for immunological research has been hindered by the unprecedented complexity of their major histocompatibility complexes. Comprehensive MHC genotyping has the potential to revolutionize the use of macaques in infectious disease research and to guide functional immunology studies. MHC-restricted cellular immune responses are key in protective immunity and resistance to infectious diseases. The comprehensive objective of this application is to increase the capacity of the ONPRC AIDS Research Expanded SPF Breeding Colony to provide genetically characterized Indian-origin rhesus macaques free of a broad number of enzootic and zoonotic agents to enhance the usefulness of the resource for cutting edge opportunistic agent and vaccine research.

项目总体概要 “扩大 SPF 恒河猴繁殖群以进行艾滋病研究” 印度恒河猴（Macaca mulatta）患上一种与人类获得的疾病非常相似的疾病 被猿猴免疫缺陷病毒（SIV）或嵌合猿猴感染时出现免疫缺陷综合症（艾滋病） 人类免疫缺陷病毒（SHIV），代表了 HIV 感染的最佳动物模型。 疫苗的开发很大程度上依赖于SIV和SHIV恒河猴模型的价值和实用性。 通过提高微生物和遗传特征的水平，模型的性能得到显着增强。 猕猴体内没有普遍存在的病毒，这些病毒是造成机会性感染的人类病毒的同源物 感染对于越来越多的艾滋病相关机会性感染模型和病毒感染至关重要 基于这些试剂的疫苗载体开发用于免疫学研究的猕猴模型。 其主要组织相容性复合体的前所未有的复杂性阻碍了它们的发展。 综合 MHC 基因分型有可能彻底改变猕猴在传染病中的应用 研究和指导功能性免疫学研究是 MHC 限制性细胞免疫反应的关键。 保护性免疫力和对传染病的抵抗力。 该应用程序的综合目标是提高 ONPRC 艾滋病研究的能力 扩大 SPF 育种群，提供具有基因特征的印度原产恒河猴，不含任何 大量的地方性和人畜共患病原体，以提高尖端资源的实用性 机会性药物和疫苗研究。