(PQC2)High-content Pathology with Confocal Microscope Arrays

(PQC2)使用共焦显微镜阵列进行高内涵病理学

基本信息

批准号：
9087173
负责人：
DAVID L RIMM
金额：
$ 62.06万
依托单位：
STANFORD UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-09-01 至 2018-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9087173
关键词：
Algorithms Appearance Architecture Area Automation Biological Preservation Cancerous Cells Cellular Phone Clinical Clinical Research Computer software Cost Savings Development Devices Diagnosis Diagnostic Ensure Evaluation Event Excision Feedback Freezing Fresh Tissue Frozen Sections Future Goals Hand Head and Neck Cancer Head and neck structure Health Healthcare Systems Hematoxylin and Eosin Staining Method Histologic Histology Histopathology Image Individual Link Malignant Neoplasms Mechanics Methodology Methods Microscope Microscopic Microscopy Miniaturization Modeling Molecular Molecular Profiling Normal tissue morphology Operative Surgical Procedures Optics Pathologic Pathologist Pathology Patients Performance Price Procedures Process Quality Control Reporting Resected Resolution Sampling Savings Scanning Series Specificity Specimen Speed Staging Staining method Stains Surface Surgeon Surgical Pathology System Techniques Technology Testing Time Tissue Sample Tissue imaging Tissues Update Visual Writing base breast lumpectomy cellular imaging cost data acquisition design detector digital imaging effective intervention fluorophore image processing image reconstruction improved interest microscopic imaging miniaturize operation prevent prospective prototype screening software development symposium tissue processing tool tumor

项目摘要

DESCRIPTION (provided by applicant): Surgical removal of head and neck cancers presents challenges to the surgeon as they seek to remove cancerous tissue, preserve normal tissues and function, and prevent disfigurement. For this purpose, assessment of tissue status at the margins is critical for effective intervention. The pathologic examination of margins for tumors and the determination of "clean margins" is currently based on traditional frozen section methods that routinely examine less than 0.2% of the tissue removed. The aims of the proposed studies are to improve the assessment of tumor margins by developing tools that effectively move the microscopic examination closer to the patient in both time and space, and that provide improved guidance for tissue selection. This will be accomplished by developing, and deploying, arrays of miniaturized microscopes that reveal tissue histopathology at a resolution approaching that of conventional microscopes, and offer large coverage of tissue samples, and high speed scanning. These arrays serve as rapid screening tools, and are enabled by developments in integrated optics, and lithographic fabrication techniques such as MOEMS (micro-opto-electro-mechanical systems), and high- resolution, high-speed digital image capture and sophisticated image-processing algorithms to accelerate acquisition and refine quality of pathology images. We propose to use these arrays at the cutting bench in the surgical pathology suite to increase the amount of tissue analyzed in gross pathology, link macroscopic inspection of tissue to microscopic examination and provide more information, more rapidly, than conventional approaches. We will construct arrays of dual axis confocal (DAC) microscopes, advance rapid fluorescent surface staining, and develop image reconstruction software to enable rapid, microscopic inspection of large surface areas of resected tissue. The arrayed dual axis confocal (ADAC) device will be approximately the size of a cell phone and will provide cellular-level images of tissue architecture and molecular signatures at 1-5um resolution, and will be applied to the pathologic assessment of tumor margins. Although this study is limited to head and neck cancers, the full potential of ADAC is substantial. The margins of resection specimens are, in many cases, inadequately sampled due to the inefficiency of current sampling methods. The ADAC device could allow comprehensive en face examination of the entire lumpectomy specimen, and will have utility in selection of tissue for processing at the surgical pathology bench. As these tools advance, the histology lab could be circumvented for many specimens, which would provide a significant cost savings to the healthcare system, and increase the speed at which pathology could be reported.

描述（由申请人提供）：手术去除头颈癌在寻求去除癌组织，保留正常组织和功能并防止毁容时给外科医生带来挑战。为此，评估边缘的组织状态对于有效干预至关重要。目前，基于传统的冷冻截面方法，对肿瘤边缘的病理检查和“清洁边缘”的测定是基于通常检查的少于0.2％的移除组织的方法。拟议的研究的目的是通过开发有效移动显微镜检查的工具来改善对肿瘤边缘的评估，并在时间和空间中更接近患者，并提供了改进的组织选择指导。这将通过开发和部署小型显微镜的阵列来实现，这些显微镜以接近常规显微镜的分辨率揭示组织病理学，并提供大量的组织样品覆盖范围和高速扫描。这些阵列用作快速筛选工具，并通过集成光学的开发以及光刻制造技术（例如Moem（Mocro-Opto-opto-eclectro机械系统））以及高分辨率，高速数字图像捕获和精致的图像处理算法来启用这些阵列。我们建议在手术病理套件的切割工作台上使用这些阵列，以增加分析的总体病理中分析的组织量，将组织的宏观检查与微观检查联系起来，并比常规方法更快地提供更多信息。我们将构建双轴共聚焦（DAC）显微镜的阵列，推进快速的荧光表面染色，并开发图像重建软件，以便对切除组织的大型表面积进行快速的显微镜检查。阵列的双轴共聚焦（ADAC）设备将大约与手机的大小相同，并以1-5UM分辨率提供组织结构和分子特征的细胞级图像，并将应用于肿瘤缘的病理评估。尽管这项研究仅限于头颈癌，但ADAC的全部潜力很大。在许多情况下，由于当前采样方法的效率低下，切除试样的边缘是不充分的采样。 ADAC装置可以允许对整个肿块切除术标本进行全面检查，并可以在手术病理台上选择组织来选择组织。随着这些工具的推进，可以为许多标本规避组织学实验室，这将为医疗保健系统节省大量成本，并提高可报告病理学的速度。