Biospecimen, Pathology, and Genomics Core

生物样本、病理学和基因组学核心

• 批准号: 8931831
• 负责人: DAVID L RIMM
• 金额: $ 20.95万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-08-26 至 2020-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8931831
• 关键词: Aliquot; Biological Models; Biology; Biopsy; Blood; Blood specimen; Cancer cell line; Cell Culture Techniques; Cell Line; Cells; Clinical; Collection; Copy Number Polymorphism; DNA; Development; Diagnostic; Epithelial; Excision; Fibroblasts; Fresh Tissue; Gap Junctions; Generations; Genomics; Genotype; Health Services Research; Human; In Situ Hybridization; K-Series Research Career Programs; Malignant neoplasm of lung; Measurement; Messenger RNA; Methods; MicroRNAs; Molecular; Needle biopsy procedure; Neoplasm Metastasis; Operating Rooms; Pathology; Patients; Plasma; Primary Neoplasm; Process; Production; Proteins; Recurrence; Research; Resources; Rho-associated kinase; Serum; Services; Source; Specimen; Surgeon; Surgical Pathology; Tissue Banks; Tissue Procurements; Tissue Sample; Tissues; Universities; Whole Blood; Work; base; cell growth; exome sequencing; immortalized cell; investigator training; kinase inhibitor; molecular pathology; personalized medicine; research study; sample collection; success; transcriptome sequencing; tumor

PROJECT SUMMARY/ABSTRACT The Biospecimen, Pathology and Genomics Core for the Yale Lung Cancer SPORE will serve as the nexus for all human biospecimen acquisition, processing and analysis for all SPORE-sponsored research as well as provide support for all non-human model system specimen molecular analytics not currently offered through Yale shared facilities. Tissue biopsy procurement will be coordinated with all interventionalists and surgeons as this occurs at the bedside or in the operating room with research tissues derived from extra passes of the biopsy needle after the diagnostic specimen has been collected or collection at the surgical pathology bench. The Biospecimen, Pathology and Genomics Core will work closely with Yale Pathology Tissue Services, the research tissue procurement service supported within the Department of Pathology (also directed by David Rimm) to obtain fresh resection specimens. Specifically the Biospecimen, Pathology and Genomics Core will: 1) coordinate the acquisition, processing, aliquoting, storage and distribution for all whole blood samples and their derivatives (e.g., plasma, serum, buffy coat) required for the described research in Projects 1-4 as well as the Developmental and Career Development Award Programs’ approved projects; 2) coordinate the acquisition, handling, storage and distribution for all lung cancer tissue sample collection required for Projects 1-4, Developmental and Career Development Awards; 3) generate conditionally reprogrammed primary lung cancer cell lines from fresh tissue samples for use in Projects 1-3; and 4) conduct molecular pathology experiments including partial support of whole exome sequencing, RNA-Seq and Copy Number Variation analysis (Projects 2 and 3); quantitative microRNA in situ hybridization (Projects 1 and 2) and other molecular pathology support as needed during the term of the SPORE.

项目摘要/摘要 耶鲁肺癌孢子的生物测量，病理和基因组学核心将作为Nexus 所有人类的生物测量，对所有孢子赞助的研究以及 为目前未提供的所有非人类模型系统标本分子分析提供支持 耶鲁共享设施。组织活检采购将与所有干预主义者和外科医生协调 因为这发生在床边或手术室，其研究组织来自额外的通过 诊断标本后的活检针头已收集或在手术病理台上收集。 生物测量，病理学和基因组学核心将与耶鲁病理组织服务紧密合作， 病理学部支持的研究组织采购服务（也由David指导 RIMM）获得新的切除规格。特别是生物测量，病理和基因组学核心将： 1）协调所有全血样品的采集，加工，等分试样，存储和分布以及 它们的衍生物（例如，等离子体，血清，血清，Buffy Coat）在项目1-4以及 发展和职业发展奖计划的批准项目； 2）协调 所有肺癌组织样品收集所需的所有肺癌组织样品收集的获取，处理，存储和分配 1-4，发展和职业发展奖； 3）生成有条件重编程的主要肺 来自新鲜组织样品的癌细胞系用于项目1-3； 4）进行分子病理 实验包括整个外显子组测序，RNA-seq和拷贝数变化的部分支持 分析（项目2和3）；定量microRNA原位杂交（项目1和2）和其他分子 在孢子期限期间所需的病理支持。