Analytical Core: Extraction, Detection, and Interpretation of PCB Congeners in Cmplex Matrices

分析核心：复杂矩阵中 PCB 同系物的提取、检测和解释

• 批准号: 9149263
• 负责人: Keri C Hornbuckle
• 金额: $ 25.74万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-12 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9149263
• 关键词: Address; Adult; Agreement; Air; Animals; Archives; Area; Basic Science; Biological; Body Burden; Breathing; Calibration; Characteristics; Chemicals; Child; Complex; Databases; Detection; Development; Electronics; Environment; Environmental Pollutants; Environmental sludge; Foundations; Frequencies; Furniture; Goals; Health; Human; Human Resources; Hydroxyl Radical; Individual; Injection of therapeutic agent; Inorganic Sulfates; Institutes; Iowa; Journals; Laboratories; Laboratory Animals; Link; Maintenance; Measurement; Measures; Metabolic; Metabolism; Methods; Molecular Weight; Mus; Paint; Parents; Peer Review; Plant Resins; Plants; Policies; Polychlorinated Biphenyls; Procedures; Process; Program Research Project Grants; Property; Protocols documentation; Publishing; Quality Control; Quartz; Rattus; Recommendation; Recording of previous events; Reference Standards; Reproducibility; Research; Research Infrastructure; Research Personnel; Research Project Grants; Research Support; Resistance; Sampling; Schools; Serum; Services; Shipping; Ships; Signal Transduction; Soil; Source; Sunlight; Superfund; Technology; Time; Tissues; Toxic effect; Training; United States Environmental Protection Agency; Universities; Unspecified or Sulfate Ion Sulfates; Urine; air sampling; animal tissue; building materials; community based participatory research; data sharing; design; high throughput analysis; innovation; instrument; instrumentation; manufacturing process; meetings; method development; photolysis; pollutant; polyurethane foam; programs; quality assurance; repaired; response; signal processing; tandem mass spectrometry; water sampling

PROJECT SUMMARY PCB analysis for research purposes is labor-intensive, requires specialized instrumentation, and depends on excellent quality control procedures. Because the Analytical Core provides the trained staff, maintains the instruments and facilities, and provides methods to assure accuracy, precision, representativeness, comparability, and reproducibility, the isrp research projects are able to make high impact discoveries, publish their results in peer-review journals, and more quickly provide scientific guidance regarding PCBs in the environment and their effect on human health. The Analytical Core supports the research of the isrp by extracting and analyzing samples for all 209 PCBs and for individual compounds in complex matrices including air samples; harbor sediment; human blood serum; urine; laboratory animal tissues; plant tissues; soils; sludge; laboratory solutions, building materials, paint, and furniture. Each year the Analytical Core will complete thousands of such measurements as well as hundreds of quality control measurements and method development measurements. The Analytical Core provides detailed quality control guidance and collaborates closely with isrp researchers to determine appropriate methods for quality assurance control. Analyses for PCB congeners in environmental matrices includes routine use of surrogate standards injected in every sample to assess and correct for analytical efficiency; internal standards for quantification; duplicate injections; laboratory blanks, calibration standards, and frequent analysis of materials with certified concentrations of PCBs. The rigor of the QC/QA protocol is unusual for PCB analysis but provides the critical foundation for discoveries in the research projects. The Analytical Core has a sustained history of innovation, starting with the development of tandem mass spectrometry for congener-specific analysis of PCBs in environmental and laboratory samples. This method enabled many important discoveries and innovative findings in the isrp, including the ability to link specific toxic properties by compound; accurate determination of the lower molecular weight compounds; and precise measurement of all PCBs. The congener-specific method used by the Analytical Core enabled the discovery of non-Aroclors in the environment. The study of these compounds, which are currently produced through some chemical manufacturing processes, has been a major area of focus since that time. Innovations planned include the development of efficient methods for routine analysis of known and as-yet unknown decay products of PCBs. Although PCBs are very persistent and resistant to decay, many PCBs can be broken down by sunlight and by metabolism in plants, animals and humans. The Analytical Core assists the research projects in measuring those decay products. .

项目摘要 用于研究目的的PCB分析是劳动密集型的，需要专门的仪器，并取决于 出色的质量控制程序。因为分析核心提供了受过训练的员工，所以 工具和设施，并提供了确保准确性，精度，代表性的方法 可比性和可重复性，ISRP研究项目能够产生高影响发现，发布 他们在同行评审期刊中的结果，更快地提供了有关PCB的科学指导 环境及其对人类健康的影响。 分析核心通过提取和分析所有209个PCB的样品来支持ISRP的研究 以及包括空气样品在内的复杂矩阵中的各个化合物；港口沉积物；人血 血清;尿;实验室动物组织；植物组织；土壤；污泥;实验室解决方案，建筑材料， 油漆和家具。每年分析核心将完成数千个此类测量以及 数百种质量控制测量和方法开发测量。分析核心 提供详细的质量控制指导，并与ISRP研究人员紧密合作以确定 适当的质量保证控制方法。环境矩阵中PCB同源物的分析 包括在每个样本中注入的替代标准的常规使用以评估和纠正分析 效率;内部标准进行定量；重复注射；实验室空白，校准标准， 并经常分析具有认证浓度的PCB的材料。 QC/QA协议的严谨性是 PCB分析不寻常，但为研究项目的发现奠定了关键的基础。 分析核心具有创新的持续历史，从串联质量的发展开始 用于环境和实验室样品中PCB的特定于同类物的光谱法。此方法 在ISRP中启用了许多重要发现和创新发现，包括链接特定有毒的能力 通过化合物的属性；准确测定较低分子量化合物；精确 测量所有PCB。分析核心使用的特定于同类物的方法可以发现 环境中的非脑子。这些化合物的研究目前是通过某些化合物产生的 从那时起，化学制造过程一直是重点的主要重点领域。计划的创新 包括开发有效的方法，用于常规分析已知和尚不清楚的衰减产品 PCB的。尽管PCB非常持久并且抵抗衰减，但许多PCB可以被分解 阳光和植物，动物和人类的代谢。分析核心有助于研究项目 在测量那些衰减产品时。 。