Research Support Core: Analytical Core

研究支持核心：分析核心

• 批准号: 10559670
• 负责人: Keri C Hornbuckle
• 金额: $ 25.99万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-12 至 2025-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10559670
• 关键词: Agreement; Archives; Carcinogens; Collaborations; Complex; Core Facility; Data; Data Analyses; Databases; Detection; Development; Environment; FAIR principles; Faculty; Food; Food Analysis; Foundations; Frequencies; Health Hazards; Human; Hydroxylation; Infrastructure; Iowa; Laboratories; Laboratory Animals; Learning Module; Measurement; Metabolic; Methods; Modeling; Neurologic; Paint; Peer Review; Pigments; Plant Resins; Plants; Polychlorinated Biphenyls; Polymers; Postdoctoral Fellow; Procedures; Protocols documentation; Publications; Quality Control; Quartz; Recovery; Reference Standards; Reproducibility; Research; Research Personnel; Research Support; Risk; Sampling; Serum; Services; Signal Recognition Particle; Soil; Source; Standardization; Superfund; Supervision; Technology; Testing; Tissues; Toxic effect; Training; Update; Water; air sampling; animal tissue; data integration; data management; data sharing; experience; graduate student; high throughput analysis; innovation; instrument; instrumentation; method development; microbial; polyurethane foam; programs; quality assurance; remediation; sharing platform; statistics; superfund chemical; water sampling

PROJECT SUMMARY: Analytical Core Polychlorinated biphenyls (PCBs) are important Superfund chemicals that are known human carcinogens also associated with neurological and metabolic disruption. The Analytical Core (AC) provides analytical services to the ISRP to facilitate high-quality research on PCBs, including data, standard operating procedures, and peer- reviewed publications that are standardized, efficient, and comparable across all ISRP matrices and projects. The AC provides instrumentation and facilities, expert staff, training, data sharing platforms, and support for method development. The AC will continue to accomplish these activities through five Specific Aims: Aim 1. Maintain analytical quality assurance (QA) standards and protocols. Accurate, precise, representative, reproducible, and comparable sample analysis is absolutely essential for all five ISRP projects and is a key AC objective. The exceptional rigor of the QA protocol for PCB analysis provides a critical foundation for discoveries by the ISRP projects. Aim 2. Facilitate high-throughput analysis in complex biotic and abiotic matrices. The AC supports ISRP projects by extracting and analyzing samples for all 209 PCBs and 72 OH- PCBs as MeO-PCBs in complex matrices, including: air samples collected on quartz filters, XAD resin, and polyurethane foam; sediment, water, and pore water samples; human serum; laboratory animal tissues; plant tissues; soils; microbial cultures; and polymers, paints, and pigments. To support new research directions within the ISRP, we will expand our capabilities to include analyses of food matrices and the ability to quantify all 837 possible mono-hydroxylated PCBs. Aim 3. Provide prioritized training and access to AC facilities. The AC, in collaboration with the Research Experience and Training Coordination Core, provides prioritized, standardized training in AC facilities and methods to graduate student and postdoc trainees, faculty, and staff from all ISRP projects. Trainees also receive assistance in method development and use of instrumentation. Aim 4. Support method development for complex matrices. The AC, in collaboration with ISRP projects, will continue to develop, maintain, and update methods for high-throughput PCB and OH-PCB extraction and analysis in biotic and abiotic samples. Separate but comparable methods are maintained across matrices to facilitate integrated data analysis. Aim 5. Maintain analytical infrastructure. The AC maintains critical infrastructure, including high-use analytical instruments, a sample database, and archives such as standard solutions, samples, and sample extracts. The AC, in collaboration with the Data Management and Analysis Core, will provide oversight to make data findable, accessible, interoperable, and reusable (FAIR) by ISRP investigators. Overall, the AC is a fully-integrated support core that assists ISRP projects to meet all four SRP mandates requiring methods for identifying human health hazards of superfund chemicals, detection, assessment of exposure risk, and remediation.

项目摘要：分析核心 多氯联苯（PCB）是重要的超级基金化学物质，也是已知的人类致癌物 与神经和代谢破坏有关。分析核心（AC）为 ISRP促进PCB上的高质量研究，包括数据，标准操作程序和同行 在所有ISRP矩阵和项目中都具有标准化，高效和可比性的审查出版物。 AC提供仪器和设施，专家人员，培训，数据共享平台以及对 方法开发。 AC将继续通过五个特定目标来完成这些活动：目标1。 保持分析质量保证（QA）标准和协议。准确，精确，代表， 可重现和可比较的样本分析对于所有五个ISRP项目都是至关重要的，并且是关键的AC 客观的。 PCB分析质量保证协议的特殊严格为 ISRP项目的发现。目标2。促进复杂生物和非生物的高通量分析 矩阵。 AC通过提取和分析所有209个PCB和72 OH-的样品来支持ISRP项目。 PCB作为复杂矩阵中的Meo-PCB，包括：在石英过滤器，XAD树脂和 聚氨酯泡沫；沉积物，水和孔样品；人血清；实验室动物组织；植物 组织；土壤；微生物培养；以及聚合物，油漆和颜料。支持新的研究方向 在ISRP中，我们将扩展我们的功能，以包括食物矩阵的分析和量化的能力 所有837个可能的单羟基化PCB。 AIM 3。提供优先的培训并访问AC设施。这 AC与研究经验和培训协调核心合作，提供了优先的， AC设施和方法的标准化培训，用于研究生和博士后学员，教职员工 来自所有ISRP项目。学员还获得了方法开发和使用仪器的帮助。 目标4。复杂矩阵的支持方法开发。 AC与ISRP项目合作，将 继续开发，维护和更新高通量PCB和OH-PCB提取的方法，以及 生物和非生物样品的分析。跨矩阵始终保持单独但可比较的方法 促进综合数据分析。目标5。维持分析基础架构。 AC保持关键 基础架构，包括高使用分析仪器，样本数据库以及诸如标准的档案 溶液，样品和样品提取物。 AC与数据管理和分析合作 核心将提供监督，以使数据可访问，可访问，可互操作和可重复使用（公平）由ISRP 调查人员。总体而言，AC是一个完全集成的支持核心，可帮助ISRP项目满足所有四个SRP 要求识别超级基金化学物质，检测，检测， 评估暴露风险和补救。