Physical Resources Core

物理资源核心

基本信息

批准号：
9140249
负责人：
Michael Anthony Moody
金额：
$ 47.79万
依托单位：
DUKE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-05-01 至 2021-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9140249
关键词：
Anatomy Animal Model Antibodies Antigen-Antibody Complex Antiviral Agents Autoimmunity Binding Biology CD32 Antigens Cell Line Cell Surface Proteins Cells Cellular biology Clinical Clinical Trials Collaborations Collection Control Animal Data Development Effector Cell Epitopes Equipment and supply inventories Exhibits FCGR3B gene Fc Receptor Fc domain Genetic Polymorphism Genotype Goals HIV-1 HIV-1 vaccine Homology Modeling Human IgG1 IgG3 Immunoglobulin A Immunoglobulin G Infection Institutes Intervention Knowledge Length Leukapheresis Lymphocyte Macaca mulatta Maps Mediating Membrane Membrane Proteins Modeling Monoclonal Antibodies Pattern Peripheral Blood Mononuclear Cell Persons Phenotype Play Predisposition Proteins Reagent Receptor Cell Recombinant Proteins Recombinants Resources Risk Sampling Signal Transduction Specificity Testing Translations Vaccines Variant Viral Viral Proteins Virus Virus-like particle Work antibody-dependent cell cytotoxicity base comparative env Glycoproteins glycosylation improved monocyte neonatal Fc receptor neutralizing antibody nonhuman primate pre-clinical preference programs prospective receptor receptor binding repository simian human immunodeficiency virus stable cell line transmission process vaccine trial

项目摘要

Core 001. Physical Resources Core The purpose of this core is to provide common physical materials needed by the projects and cores of this program in support of the overall goals. The overall Program hypothesis is that the RM model can be substantially improved for testing antibody-based interventions and vaccines through elucidation of key variables that impact species-specific FcgR-dependent effector functions (i.e. antibody epitope specificity, immune complex formation, isotype/subclass, glycosylation, and FcR genotype/phenotype. The Physical Resources Core will support Projects 1, 2, 3 (P1, P2, P3) and Core 2 (C2) and play a key role in achieving Program Goals by providing physical materials to Program components to facilitate the characterization of RM FcRs and effector cell biology across humans and RM. Drs. Moody, Saunders and Shaw have long-standing collaborations as part of the Duke Human Vaccine Institute and work together seamlessly in combining their expertise for provision of materials to collaborators. These materials include human clinical samples and corresponding materials from rhesus macaques for comparison, as well as SHIVs, and will be distributed among all three projects. The specific aims for Core 001 are as follows: AIM 1. Produce and distribute recombinant FcR proteins, membrane bound FcR proteins in virus-like particles and cell lines expressing full-length receptors. AIM 2. Produce and distribute viral proteins, RM and human antibodies, and virus stocks. AIM 3. Create and maintain a repository of human and RM PBMCs.

核心001。物理资源核心该核心的目的是提供项目和核心所需的通用物理材料支持总体目标的计划。总体程序假设是RM模型可以是通过阐明钥匙来测试基于抗体的干预措施和疫苗的大大改进影响物种特异性FCGR依赖性效应子功能的变量（即抗体表位特异性，免疫复合物的形成，同种型/亚类，糖基化和FCR基因型/表型。身体 Resources Core将支持项目1、2、3（P1，P2，P3）和Core 2（C2），并在实现方面发挥关键作用通过提供物理材料来编程组件以促进RM的表征来计划目标跨人和RM的FCR和效应细胞生物学。博士。穆迪（Moody），桑德斯（Saunders）和肖（Shaw）作为杜克人类疫苗的一部分长期合作研究所并无缝地合作，将其专业知识结合在一起，以向合作者提供材料。这些材料包括人类临床样本和来自恒河猕猴的相应材料比较以及SHIVS，并将在所有三个项目中分发。核心001的具体目的如下： AIM 1。产生和分布重组FCR蛋白，类似病毒的膜结合的FCR蛋白表达全长受体的颗粒和细胞系。 AIM 2。产生和分发病毒蛋白，RM和人类抗体以及病毒量。目标3。创建并维护人类和RM PBMC的存储库。