WOUND MIGRATION FORCE MONITORING

伤口迁移力监测

• 批准号: 8169468
• 负责人: ELIAS BOTVINICK
• 金额: $ 0.34万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169468
• 关键词: Atomic Force Microscopy; Biological; Cells; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Institution; Life; Microtubules; Mitosis; Molecular; Monitor; Optics; Pattern; Research; Research Personnel; Resources; Role; Source; Techniques; Traction; Tubulin; United States National Institutes of Health; laser tweezer; migration; transmission process; wound

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. To study the traction force transmission pattern of live cell during the cell mitosis, we will apply the traction force microscopy technique on the tubulin transfected PtK2 cell. We will use the optical techniques, including optical tweezer and laser blade and molecular biological techniques, including pharmacological inhibition to elucidate the roles of microtubules in the traction force transmission from the adherent cell to the substrate in the mitosis stage.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 为了研究细胞有丝分裂期间活细胞的牵引力传递模式，我们将在小管蛋白转染的PTK2细胞上应用牵引力显微镜技术。 我们将使用光学技术，包括光镊和激光叶片和分子生物学技术，包括药理学抑制作用，以阐明微管在牵引力中的作用，从粘附细胞从粘附细胞到底物在有丝毫的阶段。