Ultraspec III TOF/TOF 200 MALDI-TOF Mass Spectrometry Imaging System

Ultraspec III TOF/TOF 200 MALDI-TOF 质谱成像系统

• 批准号: 7595373
• 负责人: DJURO JOSIC
• 金额: $ 49.97万
• 依托单位: RHODE ISLAND HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7595373
• 关键词: Arts; Basic Science; Biochemical; Biological Markers; Cell membrane; Cells; Centers of Research Excellence; Collaborations; Communities; Complement; Complex; Computer software; Detection; Digestion; Disease; Educational process of instructing; Fostering; Funding; Funding Applicant; Gel; Goals; Health; Histocytochemistry; Hospitals; Image; Infant; Laboratories; Laboratory Research; Longevity; MALDI-TOF Mass Spectrometry; Mass Spectrum Analysis; Medical center; Mosses; NCI Center for Cancer Research; Organelles; Preparation; Proteins; Proteomics; Research; Research Personnel; Resources; Rhode Island; Sampling; Services; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; System; Teaching Hospitals; Technology; Time; Tissues; Translational Research; United States; Universities; Woman; anticancer research; base; experience; flexibility; instrument; instrumentation; mass spectrometer; medical schools; member; molecular imaging; programs; protein expression; protein profiling; public health relevance; research and development; skeletal; tissue preparation

DESCRIPTION (provided by applicant): Funds are requested for the acquisition of Ultraflex III TOF/TOF 200 MALDI- TOF Mass Spectrometry Imaging System, an up-to-date Ultraflex III TOF/TOF200 modular time-of-flight mass spectrometer together with corresponding software and accessories, to provide sample preparation for tissue molecular imaging. The mass spectrometry imaging system will also be used for protein identification, but the main propose will be the molecular imaging and detection in different protein expression between the reference (control tissue) and investigated tissue, mainly from the Major User Group (MUG). The system for molecular imaging and protein identification will significantly benefit the research and teaching endeavors of the seven NIH-funded investigators whose projects are detailed in this submission. Brown University campus- based investigators and of the five Lifespan Hospitals that are affiliated with the Brown University's Warren Alpert Medical School will also have access to this instrument. This instrument will be located in the Proteomics Core, which is part of the NIH/NCRR-funded Centers of Biomedical Research Excellence (COBRE) Center for Cancer Research Development (CCRD), and will be also used by the COBRE CCRD investigators. Two of the MUG investigators (Drs. Hixson and Moss) are also members of the COBRE CCRD program. Proteomics Core laboratory is located adjacent to Core Research Laboratories (CRL). A tight collaboration between the Proteomics Core and CRL is planned to foster optimal use of the MALDI TOF/TOF system for molecular imaging. The vast experience of the CRL laboratory and its Director, Dr. Paul McMillan, both in histochemistry and sample preparation for molecular imaging will also be an extraordinary resource to the users of this state-of-the-art instrument. Currently there are two mass spectrometers in use in the Proteomics Core. First, the QSTAR XL (Applied Biosystems/Sciex) is being used for protein identification after electrophoretic separation and in-gel digestion, or after in-gel digestion of more complex samples such as cell and organelles, especially plasma membrane extracts. The second one is the Ciphergen/Bio-Rad SELDI-TOF system, which is used for protein profiling in biomarker discovery. These instruments successfully serve a group of more than 80 costumers mostly from the Lifespan and Brown University scientific community, but also other customers from other academic communities throughout the United States. The Ultraflex III TOF/TOF 200 MALDI-TOF Mass Spectrometry Imaging System will complement and enhance the Proteomics Core mass spectrometry services, thus providing the scientific community with the flexibility and breadth to analyze proteins in numerous ways with the final goal to discover disease biomarkers and biomarkers for cellular differentiation. It is our intent that Core users' research findings from this technology will ultimately expedite proteomics-related translational research PUBLIC HEALTH RELEVANCE: The requested instrumentation will not only support the translational and basic science research of the seven Major User Group projects described in this application, but will also support investigators of five Center of Biochemical Research Excellence (COBRE), including our Center (COBRE CCRD), COBRE Center for Skeletal Health at the Rhode Island Hospital and COBRE Centers at the Brown University, Women and Infants Hospital and Roger Williams Medical Center, and will potentially benefit other investigators from Brown University and 6 affiliated teaching hospitals. Finally, the Ultraflex III TOF/TOF 200 MALDI-TOF Mass Spectrometry Imaging System will be the only instrument in this configuration and for this kind of application in the State of Rhode Island, with open access to the whole scientific community.

描述（由申请人提供）：要求资金以获取超反复III TOF/TOF/TOF 200 MALDI-TOF质谱成像系统，这是一种最新的超级反复III TOF/TOF200模块化飞行时间质谱仪软件和配件，为组织分子成像提供样品制备。质谱成像系统也将用于蛋白质鉴定，但主要提议将是参考（对照组织）之间不同蛋白质表达的分子成像和检测，主要来自主要用户组（MUG）。分子成像和蛋白质识别的系统将显着使七个由NIH资助的研究者的研究和教学努力受益，这些研究人员在此提交中详细介绍了项目。布朗大学校园的调查人员以及与布朗大学的沃伦·阿尔珀特医学院（Warren Alpert Medical School）相关的五个寿命医院也将可以使用该乐器。该仪器将位于蛋白质组学核心中，该核心核心是NIH/NCRR资助的生物医学研究卓越中心（COBRE）癌症研究开发中心（CCRD），并且还将被CRE CCRD研究人员使用。两名杯子调查员（Hixson和Moss博士）也是Cobre CCRD计划的成员。蛋白质组学核心实验室位于核心研究实验室（CRL）附近。计划在蛋白质组学核心和CRL之间进行紧密的协作，以促进MALDI TOF/TOF系统用于分子成像的最佳使用。 CRL实验室及其董事Paul McMillan博士在组织化学和分子成像的样本准备方面的丰富经验也将成为该最先进工具的用户的非凡资源。当前，蛋白质组学核心中有两个质谱仪。首先，QSTAR XL（Applied Biosystems/SCIEX）用于电泳分离和凝胶消化后，或在凝胶消化更复杂的样品（例如细胞和细胞器，尤其是质膜提取物）之后，用于蛋白质鉴定。第二个是Ciphergen/Bio-Rad Seldi-TOF系统，用于生物标志物发现中的蛋白质分析。这些工具成功地为80多种服装师提供了服务，主要来自寿命和布朗大学科学界，以及来自美国其他学术社区的其他客户。 Ultraflex III TOF/TOF/TOF 200 MALDI-TOF质谱成像系统将补充和增强蛋白质组学核心质谱服务，从而为科学界提供了灵活性和广度，以通过多种方式分析蛋白质，以许多方式与最终的目标一起发现疾病生物标志物和疾病生物标志物和疾病生物标志物和蛋白质细胞分化的生物标志物。我们的目的是，该技术的核心用户研究结果最终将加快与蛋白质组学相关的转化研究公共卫生相关性：所请求的仪器不仅支持本应用程序中所述的七个主要用户组项目的翻译和基础科学研究，但还将支持五个生物化学研究中心卓越中心（Cobre）的调查人员，包括我们的中心（Cobre CCRD），罗德岛医院的柯布雷骨骼健康中心和布朗大学妇女和婴儿医院的柯布雷中心中心，并有可能使布朗大学和6家附属教学医院的其他调查员受益。最后，Ultraflex III TOF/TOF/TOF 200 MALDI-TOF质谱成像系统将是这种配置中的唯一工具，对于在Rhode Island州的这种应用中，将是对整个科学界的开放访问权限。