Flow Cytometry

流式细胞仪

基本信息

批准号：
8084098
负责人：
JIANKE ZHANG
金额：
$ 10.02万
依托单位：
THOMAS JEFFERSON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-06-03 至 2013-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8084098
关键词：
Apoptosis Applications Grants Area Arts Basic Science Biochemical Biological Biological Sciences Biology Cancer Center Cell Cycle Cell Cycle Progression Cell Cycle Stage Cell Separation Cells Characteristics Cloning Consult Cytometry Data Data Analyses Detection Development Effector Cell Floor Flow Cytometry Flow Cytometry Shared Resource Funding Future Goals Immune Individual Investigation Kimmel Cancer Center at the Thomas Jefferson University Kinetics Laboratories Literature Methods Mission NCI Center for Cancer Research Peer Review Phenotype Play Population Positioning Attribute Price Production Property Proteins Protocols documentation Publications Publishing Reagent Reproducibility Research Research Personnel Resource Sharing Resources Role Services Sorting - Cell Movement Specific qualifier value Speed Standardization Sterility Structure of thyroid parafollicular cell Surface Techniques Time Training Universities base biosafety level 3 facility cancer therapy cell transformation cytokine fluorescence microscope genetic manipulation instrumentation member particle pathogen programs single cell analysis square foot tumor

项目摘要

The Flow Cytometry Shared Resource provides state-of-the-art cell sorting and analysis capability to the members of the Kimmel Cancer Center at Thomas Jefferson University. This shared resource is located in a dedicated laboratory space of about 600 sq ft on the 6th floor of the Bluemle Life Sciences Building, centrally positioned on the Thomas Jefferson University campus. The Flow Cytometry Shared Resource is equiped with one Cytomation MoFlo cell high-speed sorter, one Coutler EPICS Elite flow cytometer capable of sorting, one Coulter XL-MCL automated analytical cytometer, one FACS Calibur Benchtop Analyzer, one FACScan Benchtop Analyzer, two Celeron 400 workstations, one Nikon fluorescence microscope, a 30 cu ft 4¿C refrigerator, and a 10 cu ft -20¿C freezer. All units are maintained and managed by a dedicated manager/operator. The most commonly used applications are for surface phenotyping and sorting, detection of intracellular cytokines at the single cell level in mixed populations, single cell real time analysis of Ca[2+] mobilization kinetics, studies of apoptosis, cell cycle analysis, and sorting of viable cells in specific stages of the cell cycle. This shared resource allows experimental and data analysis to be performed in a timely manner and with high sensitivity and accuracy. Peer-review, funded cancer center investigators receive priority for the use of this shared resource. Based on increased capabilities for new services, the steady increase in the use of this resource by cancer center members and the recruitment of new investigators, we project that shared resource usage will increase significantly in the near future. In summary, this shared resource plays a critical role in assisting cancer center investigators as they conduct research into the biology and treatment of cancer.

流式细胞术共享资源为托马斯杰斐逊大学 Kimmel 癌症中心的成员提供最先进的细胞分选和分析能力。该共享资源位于 6 楼约 600 平方英尺的专用实验室空间。位于托马斯杰斐逊大学校园中心的 Bluemle 生命科学大楼的流式细胞术共享资源配备了一台 Cytomation MoFlo 细胞高速分选机、一台 Coutler EPICS Elite 流式细胞仪。地点能够进行分选，一台 Coulter XL-MCL 自动分析细胞仪，一台 FACS Calibur 台式分析仪，一台 FACScan 台式分析仪，两台 Celeron 400 工作站，一台 Nikon 荧光显微镜，一台 30 立方英尺 4¿ C 冰箱和 10 立方英尺 -20¿所有装置均由专门的经理/操作员维护和管理，最常用的应用是表面表型分析和分选、混合群体中单细胞水平的细胞内细胞因子检测、Ca[2 的单细胞实时分析。 +] 动员动力学、细胞凋亡研究、细胞周期分析以及细胞周期特定阶段的活细胞分类。这种共享资源可以及时、高灵敏度和准确度地进行实验和数据分析。审查、资助癌症中心研究人员优先使用该共享资源，基于新服务能力的增强、癌症中心成员对该资源的使用的稳步增加以及新研究人员的招募，我们预计共享资源的使用将显着增加。总之，在不久的将来，这一共享资源在协助癌症中心研究人员进行癌症生物学和治疗研究方面发挥着至关重要的作用。