COBRE: RIH: CORE B: PROTEOMICS: PROTEIN BIOINFORMATICS, HU GENOME, DNA

COBRE：RIH：核心 B：蛋白质组学：蛋白质生物信息学、HU 基因组、DNA

• 批准号: 7960505
• 负责人: DJURO JOSIC
• 金额: $ 61.45万
• 依托单位: RHODE ISLAND HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-10 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7960505
• 关键词: Arts; Bioinformatics; Cancer Center; Centers of Research Excellence; Chromatography; Communities; Computer Retrieval of Information on Scientific Projects Database; DNA; Electrophoresis; Fees; Funding; Genome; Grant; Hospitals; Image; Image Analysis; Immunoblotting; Institution; Isoelectric Focusing; Laboratories; Mass Spectrum Analysis; NCI Center for Cancer Research; Peptide Synthesis; Peptides; Process; Proteins; Proteomics; Research; Research Personnel; Research Support; Resources; Rhode Island; Sampling; Services; Source; Stream; System; Training; United States National Institutes of Health; base; instrument; instrumentation; mass spectrometer; member; protein purification; research and development

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Proteomics Core provides state-of-the art instrumentation and support for the research efforts of the members of this COBRE Cancer Center, other COBRE Centers located in Rhode Island and greater academic community. Our fee-based services include mass spectrometric analyses such as LC/MS/MS and SELDI-TOF mass spectrometry, electrophoretic analyses, including SDS-PAGE, isoelectric focusing, 2-D electrophoresis, immunoblot, and image analysis by use of the VersaDoc Imaging System, and protein and peptide chromatography and peptide synthesis. We also offer expertise in protein purification and characterization, and small-scale down stream processing. Our facility is located in the Coro Center at RI Hospital. In this year, a total of 23 research groups have made use of our services and facilities. Seven investigators currently use instruments in our laboratory, mainly the mass spectrometer, Ciphergen SELDI-TOF chromatographic systems for isolation, identification and characterization of their samples. Both of our mass spectrometers, a QStar XL LC/MS/MS system from ABI and SELDI-TOF from Ciphergen are in routine use. The SELDI-TOF system is also available for non-proteomics core investigators. We have also newly trained four young investigators from other COBRE laboratories to use this instrument.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 蛋白质组学核心为该 COBRE 癌症中心、位于罗德岛州的其他 COBRE 中心和更大学术界的成员的研究工作提供最先进的仪器和支持。我们的收费服务包括质谱分析（例如 LC/MS/MS 和 SELDI-TOF 质谱）、电泳分析（包括 SDS-PAGE、等电聚焦、2-D 电泳、免疫印迹以及使用 VersaDoc Imaging 进行图像分析）系统、蛋白质和肽层析以及肽合成。我们还提供蛋白质纯化和表征以及小规模下游加工方面的专业知识。我们的设施位于 RI 医院的科罗中心。今年，共有 23 个研究小组使用了我们的服务和设施。目前，我们实验室有 7 名研究人员使用仪器，主要是质谱仪、Ciphergen SELDI-TOF 色谱系统来分离、鉴定和表征其样品。我们的两台质谱仪（ABI 的 QStar XL LC/MS/MS 系统和 Ciphergen 的 SELDI-TOF）均处于日常使用状态。 SELDI-TOF 系统也可供非蛋白质组学核心研究人员使用。我们还新培训了来自其他 COBRE 实验室的四名年轻研究人员使用该仪器。